Isolation of RNA from Difficult Tissues - (Feb/18/2008 )
I tried to isolate total RNA from spleen but no result after PCR after that I used Roche kit for messenger RNA but no result also,any suggestion...........?
-desertrose-
first;
send Roche what have you done exactly,so that they can transfer your
comment to the appropriate section for troubleshooting.
till they reply;
try another kit...
i know zymosearch extracts RNA but not sure of the type of the sample.
...search for kits !!
best wishes ..
-nightingale-
The first step is to check if you got any RNA in a gel. Then you RT reaction, your PCR reaction, your primers, and your control reactions.
-chessplayer-
This is the first time to work with RNA how to do the gel?
-desertrose-