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RNA extraction from eosinophils - (Apr/17/2007 )

I am trying to extract RNA from eosinophils. I've tried the Invitrogen Micro-to-Midi extraction kit, but didn't get any results. Does anyone have any advice or ideas on how to increase my yield?



I still need help! Any ideas on how to extract RNA from eosinophils?



More details would be useful. You could try calling Invitrogen technical support; they're usually quite helpful.

-miRNA man-

i would recommend to use liquid method insterad of columns.

Basic recommendations are :
increase efficiency of lysis by using a siringe.
work always on ice and increase the lysis times up to 15'
increase the lysis buffer volume used.