Protocol Online logo
Top : Forum Archives: : Real-Time PCR

RNA from paraffin - (Jul/28/2008 )

Hello, I'm trying to isolate RNA from paraffin tissue but i'm not having results, does anybody could help me with some tips?????

-Gabyjk-

QUOTE (Gabyjk @ Jul 28 2008, 11:01 PM)
Hello, I'm trying to isolate RNA from paraffin tissue but i'm not having results, does anybody could help me with some tips?????


What exactly is the problem? Do you use a special kit for paraffin embedded tissues? Do you get any RNA out of it (how do you measure) or are you getting problems when you want to do pcr's?

-aspergillie-