Total RNA from archival tissue that is fixed and paraffin embedded (like brain t - (Aug/14/2006 )

Hi-

I'm looking for a protocol to extract Total RNA from archival brain tissue that is fixed and paraffin embedded. If you can offer any suggestions that might be please let me know.

-Thanks

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There are now some kits available that allow you to extract RNA from fixed materials. It's for formalin-fixed and paraffin-embedded tissues (FFPE). I don't know if it's suitable for brain.

They have the:
- RNeasy FFPE kit (qiagen)
- High pure RNA paraffin kit (Roche)
Also Ambion and BioVentures have those kits, but I don't know the names. Maybe the biocompare website can help you?

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