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Viral RNA detection - (Feb/08/2006 )

How would you establish whether clones contain RNA derived from a virus?

Thanks
Chrissy

-Chrissy-

QUOTE (Chrissy @ Feb 8 2006, 10:43 AM)
How would you establish whether clones contain RNA derived from a virus?

Thanks
Chrissy


Dear friend,
you can design a primer specifically for the gene fagment that you cloned-->
extract RNA form your positive clone and --> run RT-PCR.

best regards
yong

-Hadrian-