RNAi vector construction - Has anybody done it? (Apr/02/2008 )

Hello everyone,

I've recently begun working in a project and it involves the construction of an RNAi. This RNAi is meant to make a permanent and stable knockdown of a plant gene. I'm new to this kind of technology, so I require some help, if you are willing.

I have read some information on RNAi, mostly how it works. However I do not understand the difference between the hairpin RNA and the micro RNA, other than it's structure. Wich would be better for a knockdown? In addition, most if not all the info I find s aimed at animals.

In plant biotechnology, plasmid from Agrobacterium is widely used for transformation. How would it be constructed? Would this work in a RNAi insertion?

So, if somebody out there is willing to help a newbie at this, well, I can only say: thank you very much, you've saved me.

Cheers.