why low spins recommended in RNA precipitation trizol protocol? - Gibco, later Invitrogen recommend only max 12000g - why? (Feb/13/2008 )
In the TRIzol RNA precipitation protocol there are repeated warnings about using "no more than 12000g" or "no more than 7500g" (https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&objectId=47DAF8D0C311E38A951DD9C740224C67&treeNodeId=48EE00F4EFA019A020C63E2B5B3E5399). Why is that? Any ideas?
I'm used to cranking up the cooled table top centrifuge to max (25000g, 16400rpm) for DNA and also RNA precipitation in alcohol. Now I am wondering...
we use a microfuge in a cold cabinet (at 4C). and I always spin my samples at top speed. nice RNA, tha's all i can say.