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why low spins recommended in RNA precipitation trizol protocol? - Gibco, later Invitrogen recommend only max 12000g - why? (Feb/13/2008 )

In the TRIzol RNA precipitation protocol there are repeated warnings about using "no more than 12000g" or "no more than 7500g" ( Why is that? Any ideas? blink.gif glare.gif


I'm used to cranking up the cooled table top centrifuge to max (25000g, 16400rpm) for DNA and also RNA precipitation in alcohol. Now I am wondering...


we use a microfuge in a cold cabinet (at 4C). and I always spin my samples at top speed. nice RNA, tha's all i can say.

-almost a doctor-