Trizol stability - (Jan/25/2008 )
I'm about to be short of Trizol (because our lab technician did not order it even if he said he had.. but thats not the point). I went to another lab to borrow some, and they gave me a 2 year old bottle. Now my question is : has anybody used old Trizol to perform RNA extraction?
Any commentary/suggestion are welcome.
I use Trizol from a bottle that was opened in 2003, and it works perfectly!
see how you store it and how you use.
if the condition is OK, should be fine.
just worried how the owner keep that TRIZOL... has he ever forgot to put back in fridge?
or what is the purpose he use the TRIZOL for... any possibility of he contaminate the TRIZOL with his sample?
Thanks for the advice!
And the reason why they kept it is because they switched to commercial RNA extraction kits. Guess they were willing to pay more
don't forget to try a small scale procedure to see if trizol is fine.
Normally the phenol turns slight pink if oxydized.
Trizol solution is a monophasic one and as far i know doesn't seem to be affected by such process when keeped in fridge in dark bottle.
Good evening. I have some questions regarding about TRIzol reagent. From the protocol they have stated that After homogenization and before addition of chloroform, samples can be stored at -60◦C to -70◦C for at least one month. Have anyone tried that before? The second question is can I use any other reagent to replace Isopropyl alcohol during RNA precipitation?
Thank you guys!!
Thank you guys!!
Stored at -60◦C to -70◦C for more than one month is OK.
Ethanol could be used instead of isopropanol, however, 2-2.5 volumes of the supernatant of ethanol should be used. You may see Molecular Coloning: A Laboratory Manual (2nd edition) for more details about ethanol/isopropanol precipitation of nucleic acids.
But why would you want to change isopropanol?