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DIG RNA PROBES - (Jul/12/2001 )

I am Having a problem with severe filter background on a DIG labelled RNA probe.No matter what conc:of probe I use the background is really bad.Can anyone suggest a cause and solution to my problem.


How are you labelling your RNA i think you mean cDNA from RNA right? Back ground problems tend to arise when your membrane gets dry in the CDP-STAR™ stage. Try increasing the amount of volume you use. Also, try a more stringent SDS % solution which may help to reduce the peskie background.