Protocol Online logo
Top : Forum Archives: : Immunology and Histology

Quality of RNA by spectrophotometer - (Jun/27/2005 )

The A260/A280 ratio of my RNA purification is often >2 sometimes even >3. Does anyone know the cause of this and how to ommit it?

The RNA is purifyied from barley plants with the RNAeasy kit from qiagen with an on-collum DNase treatment. The RNA is going to be used for real time RT-PCR. Is the high ratio a problem for this application?


there were discussions
and also
and finally part of discussion


Thanks, I'll try and see if there are a good idea in these discussions


I've solved the problem. It seems that reducing the amount of starting material does the trick. The ratio is now 1,9 without any decline in yield. rolleyes.gif