where is the best place to perform the RNA extraction work and the relevant? - (Aug/16/2008 )
I have never done the RNA work before. Currently there is limited space in our lab and I want to know it is acceptable to conduct RNA extraction and the relevant work just on the bench?
Or do I need to perform them in a fumehood?
And is there anything else I have to pay attention to carefully? Thanks a lot!
Besides, I know I have to use RNase free solvents and consumables as much as possible. For those non-disposable consumables, the right way to get rid of RNase is autoclavation and baking them in a drying oven at 150 degrees for at least 4 hours, is that correct? I appreciate any comments and suggestions!
Ambion are a very useful resource when doing RNA work. This should help you out.
Thanks, stevo. A lot of information about the RNA work on that website.