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Isolation of total RNA from whole blood - How much Trizol do I need? (May/10/2007 )

Dear all,

I want to isolate the total RNA from whole blood. How much Trizol should I use? eg. 1ml blood, do u think 0.8ml trizol is enough? I'm afraid the quality of RNA will be affected if the amount of Trizol is not enough.




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Hi, I routinely use 250µl EDTA blood, 500µl Trizol and later 100µl Chloroform. It also works if you doubel all amounts. For 1ml blood you should use 2ml Trizol.



Dear All,

Thx so much!!!! biggrin.gif