Nuclear RNA knockdown with siRNA - is it possible? (Feb/09/2005 )

Hello,

I'm trying to knockdown the amount of a nuclear RNA. Does the Dicer-mediated degradation of the targeted RNA occur in the nucleus or in the cytoplasm??

If it occurs in the cytoplasm, does polyadenylated nuclear RNA get exported to the cytoplasm before it is localized to the nucleas??

Is knocking down a nuclear RNA feasible?

Thanks a lot,
Nick

There is a very recent paper reporting RNAi works in the nucleus. The abstract is here
http://www.ncbi.nlm.nih.gov/entrez/query.f...t_uids=15643423

my experience is that in the nucleus, antisense works a lot better than RNAi.

hi
a recent paper may help you :
http://www.ncbi.nlm.nih.gov/entrez/query.f...t_uids=12679785
they worked on mouse embryo


and you may have a look at this topic
http://www.protocol-online.org/forums/inde...?showtopic=5037

hope that helps
Fred

this paper may help you

http://www.ncbi.nlm.nih.gov/entrez/query.f...335&query_hl=10

a recent paper may help you :
http://www.ncbi.nlm.nih.gov/entrez/query.f...t_uids=12679785
they worked on mouse embryo

For mammalian cells, try antisense oligo to knock down nuclear-retained RNA.