RNasing - (Aug/08/2001 )
I am working with a set of primers which require RNase prior to PCR. I have made several attempts however,in all cases I have also lost most if not all of the DNA. Does anybody have any suggestions or knowwhat conc. the RNase should have of the final volume.
This may be a stupid question but is your RNase DNase free? Are you using more or less than 10ng/ul?