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RNasing - (Aug/08/2001 )


I am working with a set of primers which require RNase prior to PCR. I have made several attempts however,in all cases I have also lost most if not all of the DNA. Does anybody have any suggestions or knowwhat conc. the RNase should have of the final volume.




This may be a stupid question but is your RNase DNase free? Are you using more or less than 10ng/ul?