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amplificatin of bacterial total RNA - (Mar/06/2008 )


I am looking for a protocol or some hints how to perform
cDNA synthesis and amplification by using total RNA from bacteria.

Thanks in advance



One kit for isolation of bacterial RNA is here:

cDNA Synthesis can be done using Promega AccessQuick RT-PCR System (catalog# A1701)

I personally swear by Promega. I've used their RT-PCR kits before and have always had great results. They are pretty simple and don't take huge amounts of time either.