RNA Isolation from bacteria - (Oct/05/2004 )
It is well known that half life of bacterial RNA is very short and unstable. I am doing macroarray experiment with bacteria,and it is very important to prevent RNAs from being degraded during collecting cell. I tried it with 5% cold phenol/industrial-grade alcohol,but not sure whether it will work well .Would anyone there like give me some suggestions on it.
Thanks in advance.
weimin
-weimin42-
we have had good experiences with RNAlater from Qiagen. maybe worth a try for you?
mike
-jadefalcon-
Try Qiagen kit Bacteria later, works great
-huasa7-