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RNA Isolation from bacteria - (Oct/05/2004 )

It is well known that half life of bacterial RNA is very short and unstable. I am doing macroarray experiment with bacteria,and it is very important to prevent RNAs from being degraded during collecting cell. I tried it with 5% cold phenol/industrial-grade alcohol,but not sure whether it will work well .Would anyone there like give me some suggestions on it.

Thanks in advance.

weimin

-weimin42-

we have had good experiences with RNAlater from Qiagen. maybe worth a try for you?

mike

-jadefalcon-

Try Qiagen kit Bacteria later, works great

-huasa7-