RNA isolation fromparaffin-embbed tissu - (May/16/2001 )
I tend to isolate the total RNA from the formalin fixed ,paraffin-embedded specimens,then do RT-PCR with the sample.Could you inform me a relatively easy and reliable method to do this work.And I would rather use the TRIZOL@Reagent (life technologies) to isolate the total RNA,as we used in the fresh tissu RNA isolation.
A company called GENTRA SYSTEMS, INC have some products for isolating RNA from paraffin embedded tissue. It is called PUREscript RNA isolation kit. You may want to call their technical assistance for more help. The number is: 1-800-866-3039. Good luck!