Protocol Online logo
Top : Forum Archives: : Botany and Plant Biology

detergent in RNA extraction - (Apr/07/2006 )

Hi all
What the strongest detergent used in plent cell lysis in RNA extraction
and what the most used polyphenol absorbant used

-Najib-

Generally sodium dodecyl sulphate is used, or N-laurylsarcosine, for the detergent

-John Buckels-

QUOTE (Najib @ Apr 7 2006, 10:42 PM)
Hi all
What the strongest detergent used in plent cell lysis in RNA extraction
and what the most used polyphenol absorbant used



detergent could be SDS

-coffee-

QUOTE (John Buckels @ Apr 10 2006, 03:00 PM)
Generally sodium dodecyl sulphate is used, or N-laurylsarcosine, for the detergent

CTAB can also be used as detergent

-Hegde-