How can i measure concentration of RNA?

Which solvent to dilute it?

And which solvent to store it and at what temperature?


Thanks.

hi
RNA concentration should be measured by diluting dried RNAs in DEPC treated water, heating them 10' at 50° and OD at UV260nm.
This topic may give adds.
this topic deal part with RNA OD260/OD280 > 2.

10' is 10 minutes, just as 10" is 10 seconds

This is based on the imperial system of degrees (small superscript circle), minutes ('), seconds (") of arc for a compass direction/latitude-longitude, though the system is also used for time as above, and for distance with feet being (') and inches (").

TE is sometimes used to inhibit RNases and make the RNA more stable. The Tris buffers the pH to approx 8 at wich point RNA is more stable in solution and the EDTA chelates and metal ions that RNases use as cofactors, hence inhibiting their action.

You should spec your sample against the same liquid you used to dissolve it, i.e. if you use water to dissolve, blank the spectrophotometer with water.

Bob

hi
heat them 10minutes at 55degrees celsiuis improve the efficiency of RNA resuspension and accurates the OD measurment.
i was told to respspend RNA in ddH2O, and i think it's because this colleague make transcriptions in vitro with her RNA.
I think that for a norther analysis TE won't interfer anyway.

fred