Isolation of a specific mRNA? - RNA pull down (Apr/25/2007 )
Does anyone know of a protocol to isolate or pull down a specific mRNA from cells. For example maybe you can use a tagged cDNA or antisense RNA bound to beads to pull out or isolate the complementary message?
hope you can help
Wages JM Jr; Wages GM; Matthews P; Weller D; Summerton J. Affinity purification of RNA: sequence-specific capture by nonionic morpholino probes. Biotechniques. 1997 Dec;23(6):1116-21.
I never tried it but I think you can make a biotinylated antisense of your mRNA, bind it on streptavidin beads and purify like any other chromatography ..