Easy protocol for purification of in vitro transcribed RNA oligonucleotides? - (Jan/24/2006 )

Hi all,

I am using the T7-RNA-Polyermase system to generate my siRNAs in vitro. The systems works fine, I have switched to using single components (instead of kits), however I haven't figured out an easy way to purify my RNA oligonucleotides after transcription. Phenol/chloroform extraction works, but it is a tedious and long procedure. I have tried to find protocols on using affinity spin columns on the net, but I couldn't find any. Are you aware of any protocols that use affinity spin columns (maybe for PCR-product purification ...) to purify siRNAs or RNA oligonucleotides?

That would be of great help.

Thanks,

tevi

alcoholic precipitation should work.