RNA extraction of a very, very small tissue sample - (Nov/21/2006 )
Hi I have started with RTPCR 2 months ago. I have performed well the experiment but the problem is that now I have to make a RNA extraction from a tissue of "ONLY 0.02 mg" and I have no idea of how I can process this amount of tissue.
If someone could give me some idea I would be very grateful.
Thanks a lot in advance
I remember a kit for purify RNA from microdissected slice which is much more small sample, check/google literature related to Laser Capture Microdissection (LCM)
i would use trizol which allow you to recover all RNA and by the fact you keep the phenolic phase, you may able to recover the nucleic acids if needed by butanol precipitation of the whole phenolic phase