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reprobing small RNA blots with U6 - (Oct/09/2007 )

Small RNA blots are often reprobed with U6 (or other snRNA) probes for loading control. These should give a signal of about 80-100nt, which is fairly well distinct from a miRNA signal, so, is it necessary to strip the blots before reprobing? Wouldn't even be possible to hybridize with a mixture of a miRNA probe and the U6 probe at the same time?

-andrea massimo-

I have the same question

And now , I reprobe u6 without striping the membrane .the results looks good , as you said,the length is significantly different!

I didn't use 2 probes at same time .Anybody tried, please tell us the results,hur?