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Microarray RNA extraction - (Jul/21/2008 )

Hallo everybody,
I am doing microarray RNA extraction with a very good cell count but I found for actual microarray quality RNA I should give a Optimized DNAse treatment,but I am confused in which step shall I give DNase treatment can anybody has anything to say about it.Think before answaring because I am an experienced person so dont give Rubbish answar. huh.gif

-cy finder-

My steps were
1. Isolate RNA
2. Clean it up using Qiagen kit
3. DNase treatment
4. Follow Affymetrix protocol for labeling

Hope this helps.

-Jiang M-


First isolate RNA then give DNAse treatment and finaly purifiy RNA using Qiagen column chromatography. Same things i also using for my microarray experiments.
But i using Trizol methods in some sample after RNA isolation i purified RNA using RNesy Purification kit after purification no need to DNase digestion (confirmed by running QC samples on bioanalyzer for quality checking)



QUOTE (Jiang M @ Jul 21 2008, 04:16 AM)
My steps were
1. Isolate RNA
2. Clean it up using Qiagen kit
3. DNase treatment
4. Follow Affymetrix protocol for labeling

Hope this helps.

-awadh-