RNase decomtamination - (Jul/08/2007 )
Hi all. Simple question. I need to decontaminate some ceramic mortar-and-pestle sets to grind up bone tissue for RNA extraction. I was going to bake them @ 400`C overnight (~16hours). Do you think this this long enough or hot enough? Cheers-JAH
I used to wash them with DEPC water and autoclaved.
I'm used to work with RNA and I usually put all the glassware in a oven at 200 °C overnight and it is sufficient to remove any RNase... so 400 °C ! I think it's ok
Good luck !
Yea.. I agree with Hyland, use DEPC autoclaved water. Perhaps just try out the RNase zap free but I think it is quite expensive.
i won't use autoclaved DEPC water but non autoclaved DEPC water, as the aim is to block RNases.
Then just autoclave your material.
I'm using RNAse AWAY from invitrogen. It removes rnase and DNA contamination.
Baking is the easiest. 180 oC for a few hours does the job. I agree with fred_33 on the depc-water, and _then_ autoclaving.