Series dilution of RNA or cDNA for qPCR standard curve? - (May/25/2006 )
Do you use serial dilution generated from directly RNA or do you dilute cDNA that is synthesized from 1 ug mRNA?
-molcyt-
QUOTE (molcyt @ May 25 2006, 02:41 PM)
Do you use serial dilution generated from directly RNA or do you dilute cDNA that is synthesized from 1 ug mRNA?
I use serial dilution of RNA generated In-vitro transcription.
This will give you a more acurate amount of your standard.
best regards
-Hadrian-