High throughput RNA extraction - (Dec/16/2005 )

Is there any suggestions on high throughput RNA extraction? It should be at least 96 well plate based method. I am aware of the kits from qiagen, gentra and ambion. Is there any modified method that is more cost effective?

Thanks.

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hai,
well, using TRIzol reagent actually helps you to obtained high yield of RNA rather than using those expensive RNA extraction kits.
good luck

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But TRIzol is not that friendly to be used for high throughput extraction.

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ooo..ok..i guess u have to use an expensive method...i can't think of usual way to produce high throughput RNA extraction other than using the commercialized RNA extraction kit..sorry..

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The ChargeSwitch RNA from cells kit can be used in a 96 well format, manually or automated

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Does it work for plant tissues?

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jeff.quo, we are using a 96-well plate extraction vacuum method from BioRad that works very well with plant tissue, and is much cheaper than Qiagen.

http://www.biocompare.com/productdetails/1...om-Bio-Rad.html

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No

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How much for each sample?

Do you know any 96 well plate for both DNA and RNA isolation?

Thanks.

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it works out to ~2.10 US per sample. i don't know of any plate extraction system designed to work for both DNA and RNA.

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