RNAi or Antisense oligos for inhibition in vitro and in vivo - (Sep/20/2007 )
I'm wanting to do some inhibition work but i'm not sure which of the techniques I should use. I know RNAi is the most widely used at the moment but i have read somewhere about there being problems using it in vivo and sometimes in hard to transfect cells. Has anyone got any suggestions?
A comparison of siRNA with several sorts of antisense is the topic of the following paper:
Summerton J. Morpholino, siRNA, and S-DNA Compared: Impact of Structure and Mechanism of Action on Off-Target Effects and Sequence Specificity. Med Chem. 2007;7(7):651-660
Getting the molecules into cells is a challenge for any of these gene knockdown structures. Techniques are improving, and there are some good choices for each of the molecules (though what works well for siRNA might not work well for Morpholinos and vice versa). The problem becomes even more challenging in vivo, but again techniques are improving. See, for example, Hong Moulton's work with peptide-conjugated Morpholinos: PubMed search "Moulton HM":