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problems with RNA bands - on agarose gel (Oct/30/2007 )

after i run as usual with agarose gel, i discover a weird things on my gel...
in fact, for RNA works, the gel shld only have the 18S and 28S ribosomal RNA bands and some lower molecular weight smear...
but for my stuff, i got a band above the 28S RNA band, around 1cm above from the gel picture...

at first, we thought it might be DNA contaminant, but after run with PCR, it doesnt show any product..
and when RNase A to treat it, the band disappear also...

so, we now in the doubt whether it is also the RNA band...

do u all ever face this kind of problem??
thanx for help...

-parami-

QUOTE (parami @ Oct 30 2007, 01:42 AM)
after i run as usual with agarose gel, i discover a weird things on my gel...
in fact, for RNA works, the gel shld only have the 18S and 28S ribosomal RNA bands and some lower molecular weight smear...
but for my stuff, i got a band above the 28S RNA band, around 1cm above from the gel picture...

at first, we thought it might be DNA contaminant, but after run with PCR, it doesnt show any product..
and when RNase A to treat it, the band disappear also...

so, we now in the doubt whether it is also the RNA band...

do u all ever face this kind of problem??
thanx for help...


It must be genomic DNA. Add some DNase I and any buffer and it should be gone in a few minutes.

-chessplayer-