RNA isolation from frozen tissue sections - Which Kit do YOU use? (Apr/12/2006 )
I am trying the ZYMO Pinpoint Slide RNA Isolation System 1. The manual says 1 mm2 of a 10 micrometer section gives enough RNA for subsequent RT reaction and quantitative RT PCR. I say thats a lie.I used up to 4mm2 and did not get detectable amplification.
What is your opinion? Please share your experiences!
I used qiagen mRNA isolation kit, qiagen RNA isolation kit for tissues. Both worked fine.
Homogenization of tissue is very critical step. Take care at that level.
Try this method also
Thanks a lot! I will try your suggestions in the next couple of weeks.