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small RNA (100-500bp) - (May/21/2006 )

hello everyone,

I am working on parasites infecting RBCs.
I want to isolate small RNA of parasites from blood
Can anyone pls send me the protocol for isolation of small RNA from human RBC.
thanking in anticipation.



isolate RBC
Do the trizol / tri reagent procedure
Or, you can do a column based kit, but i don't have too much experience with them and RBC.