Does RNA degradate in the refridgerator (4C)? - (Nov/18/2005 )
I left my precious RNA accidentily in the refridgerator at 4 degrees C instead of at -20 in the freezer. Do you think that it degraded in just one day?
-babybug-
Run a gel of it, or do an RT-PCR that in your hands always works (and include a positive control).
-vairus-
What is it dissolved in and how did you purify it? That will determine how clean it is and how stable it is.
-tap14-
RNA dssolved in formamide is more stable than in nuclease free H2O
-fred_33-
I think the RNA in nuclease-free water should be kept at -70 instead of -20, right?
Have you run a gel to check? Could you please just let me know whether it degraded in just 1 day?
-sewyeng-
It didn't degrade. Able to run PCR and get the bands we are looking for.
-babybug-