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help for total RNA from skin mice - (Mar/23/2006 )

sad.gif How to extract total RNA from skin of mouse?
how to obtain a good output and a ratio DO260/DO280 corret ?
thank you AND best regards laugh.gif


i would say that kits and liquid preps are ok....
Due to facility to adjust lysis solution volume and the fact you can't get overload, i would say trireagent is better. But it's as you want.


I have successfully extracted RNA from monkey skin several times using Trizol and a polytron. A good fast grind of the tissue is the trick.