RNA isolation stationary phase - (May/01/2007 )

Hello,

I am optimizing the RNA isolation of Rhizobium etli. I've got good results with cultures in exponential phase, using RNeasy Kit (Qiagen) and Total RNA purification (Promega) and Trizol (Invitrogen). But the yield of cultures in stationary phase is drastically less.
Can this only be resolved by using a larger volume of culture? Or try a other lysis-method?

Maarten

you may try larger volumes.
But the amount of lysis solution (either in kit or liquid ones) should be increased. Typical is to use 2fold trizol as recommended.

Second : did you try a carrier such as glycogen or PEG ?