RNA stability and Long term storage of homogenate in Trizol or similar reagent - (Mar/10/2006 )
I am homogenizing my human fat Biopsies in Trizol or TRI reagent. My intention was
to store the homogenate at -80c. I noticed however in the product protocol
it says homogenate is stable for "at least 1 month", I intended to
store the samples for longer than this, does anyone have any information or experience
on stability of RNA after long term storage in Trizol as a homogenate.
Just as a note: Fat Biopsies cannot be stabalized for long term storage with reagents such as RNAlater, the fat prevents penetration.
I've read on the invitrogen website, and have also been informed directly by invitrogen tech support, that the homogenate is good for at least 1 year at -70°C. I actually freeze mine at -80°C, and haven't had any trouble with RNA integrity. Please note that with my own experience, the longest my samples have been stored has been about 3-4 months.
Ok...I tried to get the link to the specific page to work, but it only brings you to the general FAQ page. If you type in "trizol" and do a search under FAQ's, they have a discussion of stopping points and for how long the RNA can be stored at each stopping point.
Please let me know if you can't find it! Good luck-
for long term storage, i prefer to store directly a snap frozen cell pellet rather than store the homogenates. It's suitable for more than 6month without loosing RNA quantity and quality.