Aniline cleavage of RNA - (Jun/06/2008 )
Hi all, I have a question about aniline, or rather how to prepare it. I work with ricin a-chain variants and am attempting to get a gel picture of the cleavage fragment that occurs after chain scission by aniline. Most protocols call for aniline acetate ph 4.5 but I have been unable to find this chemical for purchase or find a recipe for it. If anyone has one that would be great. I have purchased aniline from fisher.
adjust the pH of the aniline with acetic acid.
will become acetanilide over time so make it fresh when you need it.
(info from merck index).