Protocol Online logo
Top : Forum Archives: : Molecular Biology

rRNA in insects - Information about 18S, 5.8S, ITS1 & ITS2 (Oct/15/2003 )

I need information about rRNA in insects, like organization and secondary structure. I read an ancient paper (~1979) where is explained this organization in Drosophila and they say that ITS region is composed by 18S-ITS1-5.8S-ITS2a-2S-ITS2-28S (in this order). The typical organization is more simple, like 18S-ITS1-5.8S-ITS2-28S. Searching in databases I found that this especial organization is present in Diptera (only ??).
Other cuestion is how can I know where start and where finish each one of the rRNAs
(18S, 5.8S and 28S) because these are not genes and they dont have an AUG. I started comparing with some sequences in databases, but my sequences are a bit different, for example at the start of 5.8S (around four nucleotides).

If somebody can help me I'll be thankful


these organization are typical for all eukaryotic, you can read the paper of Hillis & Dixon (The Quarterly review of Bology, 66 (4): 411-453), is not so recent (1991) but it can be usefull. the journal is online but if you don't find it let me know that I'll send it to you.