Tissue storage for RNA isolation - (May/22/2005 )
Please, give your thought! Thanks in advance.
When we first do an isolation then we first have to do a mini prep to test for the quality before we do a maxi or a bigger prep of a sample. MY question is i have to do total RNA isolation from a tumour tissue how can i just isolate a little RNA from this tissue and then freeze the rest of the tissue for later use? Would not this Freeze-thaw circle damage the tissue for later use?
well, does this mean that noone has ever isolated total RNA from a tumour tissue before? Would the thaw and freeze circle affect the quality of it for later use?
Hope very much for an idea!
RNA in tissue cannot stand repeat freeze-thaw cycles. You can store tissue in a solution called RNAlater (from Qiagen and Ambion) or divide your tissue into small pieces and store them in liquid nitrogen.
so we do always make miniprep of a sample before doing a maxi/bigger prep for the experiment?
If you are sure your method works, you don't need to do 'miniprep' for every sample.