Resuspend RNA in TE or ddH2O? - (May/24/2008 )

I'm isolating RNA from human cancer cells. I've isolated RNA from plant tissue in the past and in the end I always resuspened my RNA pellet in RNAse free ddH2O for storage and PCR. The protocol I was given for this project says to resuspend in TE.

My first question is what's the difference? Is one better than the other? Is there a risk in using TE?

Second, what the standard TE recipe?

Thank you!

p.s. New to this lab and not alot of guidance... don't know what I'd do without this site!

-Spoony-

hello Spoony,
i use ddH2O for resuspending my RNA pellet and storing it in the freezer.

here is a link,think it would be useful :-
http://www.ambion.com/catalog/CatNum.php?7000

-nightingale-

I always resuspend RNA in ddH2O. TE gives added security against nucleases, but may inhibit dowstream reactions (such as reverse transcription).

-beenyg-