Resuspend RNA in TE or ddH2O? - (May/24/2008 )
I'm isolating RNA from human cancer cells. I've isolated RNA from plant tissue in the past and in the end I always resuspened my RNA pellet in RNAse free ddH2O for storage and PCR. The protocol I was given for this project says to resuspend in TE.
My first question is what's the difference? Is one better than the other? Is there a risk in using TE?
Second, what the standard TE recipe?
p.s. New to this lab and not alot of guidance... don't know what I'd do without this site!
i use ddH2O for resuspending my RNA pellet and storing it in the freezer.
here is a link,think it would be useful :-
I always resuspend RNA in ddH2O. TE gives added security against nucleases, but may inhibit dowstream reactions (such as reverse transcription).