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Resuspend RNA in TE or ddH2O? - (May/24/2008 )

I'm isolating RNA from human cancer cells. I've isolated RNA from plant tissue in the past and in the end I always resuspened my RNA pellet in RNAse free ddH2O for storage and PCR. The protocol I was given for this project says to resuspend in TE.

My first question is what's the difference? Is one better than the other? Is there a risk in using TE?

Second, what the standard TE recipe?

Thank you!

p.s. New to this lab and not alot of guidance... don't know what I'd do without this site!


hello Spoony,
i use ddH2O for resuspending my RNA pellet and storing it in the freezer.

here is a link,think it would be useful :-


I always resuspend RNA in ddH2O. TE gives added security against nucleases, but may inhibit dowstream reactions (such as reverse transcription).