help with buffer 5.1 for RNA extraction - (Jan/17/2006 )

Dear all,

anyone who knows how to prepare the buffer 5.1 for RNA extraction? Does that all the reagent need to be RNase free? Where can we get them?

Thanks a lot

-banana-

Which supplier and which kit?

-John Buckels-

I want to make it by myself. like buying EDTA, sodium acetate et al. What is a good vender ?

Thanks

-banana-

You will have to define what exactly is buffer 5.1

If I asked you "how do I make buffer XF8?" you wouldn't know what I was talking about. This is what is happening here

-John Buckels-

The buffer which you fill to keep the phenol from oxidize. It has EDTA, sodium acetate, glacial acetic acid, all of these need to be RNase free.

-banana-

I doubt the reagents need to be RNase free as the phenol will strip away any proteins, however I tend to buy mol bio grade chemicals for all my work

-John Buckels-

Thanks a lot!

-banana-