Re: How to extract RNA fromFormalin fixed tissue - (Mar/08/2001 )

I need a good protocol for nonradioactive in situ hybridizations. I will be using liver tissue sections.

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If you can specify what probe are you going to label and what nucleotide sequence are you going to detect I may provide you with some information

Longcheng LI

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Hi,

There is a great in depth article in the Boehringer (now Roche) newsletter, Biochemica. You can find it at:http://biochem.boehringer-mannheim.com/Pro.../no1_98/p10.pdf

Another key reference is the one by Schaeren-Wiemers & Gerfin-Moser (1993) Histochemistry 100:431.

Good luck!

-Jonathan Lytton

Check Clontech's SMART system. It is really reliable. Good luck