Re: How to extract RNA fromFormalin fixed tissue - (Mar/08/2001 )
I need a good protocol for nonradioactive in situ hybridizations. I will be using liver tissue sections.
If you can specify what probe are you going to label and what nucleotide sequence are you going to detect I may provide you with some information
There is a great in depth article in the Boehringer (now Roche) newsletter, Biochemica. You can find it at:http://biochem.boehringer-mannheim.com/Pro.../no1_98/p10.pdf
Another key reference is the one by Schaeren-Wiemers & Gerfin-Moser (1993) Histochemistry 100:431.
Check Clontech's SMART system. It is really reliable. Good luck