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Protein Related Discussions
  1. Validating Recombinant Protein - (reply: 1)
  2. Protein active site size calculation - (reply: 1)
  3. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
  4. protein G - (reply: 1)
  5. Question: Removal of Bacteria from Protein to achieve 100cfu/gm - (reply: 5)
  6. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
  7. the same construct, two stable cell clones with different protein size - (reply: 2)
  8. Western Blot: protein band did not appeared at the correct location - (reply: 2)
  9. I want to separate two proteins having mw of 16.8 and 16.78 kDa. how can i accom - (reply: 2)
  10. epitope tagging of protein - (reply: 1)
  11. Finding potential binding targets for a transcriptional regulator protein? - (reply: 1)
  12. i am trying to get rid of unexpected protein band by centricon - (reply: 3)
  13. Choosing MWCO For Dialysis Membrane - (reply: 3)
  14. Making a fusion protein - (reply: 1)
  15. CO- IP for nuclear protein - (reply: 4)
  16. Cloning a fusion protein, do I remove the ATG codon. - (reply: 1)
  17. Resuspension of Protein after TCA Precipitation - (reply: 1)
  18. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
  19. how to interpret or present elisa result for IgA in pig obtained from 120 saliva - (reply: 3)
  20. Ladder and proteins do not migrate into stacking gel - (reply: 5)
  21. Trichloroacetic Acid precipitation of proteins - (reply: 2)
  22. How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vector - (reply: 1)
  23. How to increase protein expression from in vitro transcribed mRNA - (reply: 1)
  24. Protein degradation SDS PAGE - (reply: 5)
  25. Protein extraction from RNAlater - (reply: 1)
  26. protein expression with and without induction - (reply: 1)
  27. Puromycin resistance protein expression and stability - (reply: 5)
  28. Prion protein - (reply: 3)
  29. Please help me :Protein expression and purification - (reply: 1)
  30. Calculating molar ratio & dilution of protein cluster solution - (reply: 9)
  31. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
  32. genotype of e coli strain for protein expression - (reply: 2)
  33. Blocking agent in western blotting of histone proteins - (reply: 1)
  34. Problems with detection of protein expressed in yeast - (reply: 1)
  35. How do I go about making a set of protein standards for protein quantification v - (reply: 2)
  36. Protein Refolding - Precipitation during dialysis - (reply: 3)
  37. What is the RNA:Protein ratio for ONE gene over different RNA levels? - (reply: 1)
  38. Protein Glycosylation and SDS-PAGE - (reply: 2)
  39. Using a mouse antibody for IP - should I use Dynabeads with Protein G or with sh - (reply: 1)
  40. protein turn-over - (reply: 8)
  41. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
  42. protein structure visualisation - (reply: 3)
  43. heme protein precipitation - (reply: 4)
  44. how much purify protein nedded for IP - (reply: 9)
  45. lyophilized protein dilution - (reply: 6)
  46. Protein isolation for tissue stored in RNAlater - (reply: 1)
  47. regarding protein purification using 8m urea and sds page - (reply: 2)
  48. Question about IgG & IgM cross reactivity - (reply: 1)
  49. detection of metal in protein - (reply: 4)
  50. Do you go by 'total protein' or 'tissue weight'? - (reply: 1)
  51. Protein Purification from mammalian cells - (reply: 1)
  52. Non-denaturing buffer to extract nuclear protein - (reply: 1)
  53. Immunofluorescence for detecting proteins in cytoplasm - (reply: 6)
  54. How many ways protein protein interactions are regulated? - (reply: 1)
  55. Protein quantification after TRIzol Extraction - (reply: 2)
  56. Protein fragment is alwasy degraded post transfection 48 hrs - (reply: 2)
  57. Can an protein be tolerized against if it doesn't have an MHCII epitope? - (reply: 1)
  58. in what technique can i use to detect specific proteins - (reply: 5)
  59. Modeling of a protein with disulfide bond - (reply: 7)
  60. Protein is present, but not detected by antibody - (reply: 4)
  61. fluorescent tag on membrane protein - (reply: 2)
  62. Is it possible to attach any protein with GFP and express together? - (reply: 6)
  63. loading control issue in blot with H9c2 cell protein, pleeeeeasee help - (reply: 2)
  64. Have I used too much protein in my ELISA? - (reply: 1)
  65. High MW IgG signals in IP/co-IP with protein G - (reply: 1)
  66. Pulldown of polyubiquitinated proteins - (reply: 1)
  67. PCR that leads to protein synthesis - (reply: 18)
  68. membrane protein expression - (reply: 2)
  69. purified protein binds cell surface. how to be sure it is not an artifact? - (reply: 1)
  70. structure prediction of a Protein with disulfide bond? - (reply: 4)
  71. Ligand-controllable degron (Shield-1) for tunable protein stability - (reply: 1)
  72. identification of major protein; public 2D/mass-spec databases? - (reply: 2)
  73. having problems with p-erk antibody and other phosphoproteins antibodies - (reply: 1)
  74. SNP effect on protein binding affinity - (reply: 5)
  75. Help, protein ladder didn`t separate - (reply: 3)
  76. Protein concentration and extinction coefficient - (reply: 2)
  77. N-terminal epitope tag - keep or remove ORF start codon - (reply: 1)
  78. Protein secretion wtih pFuse expression system in 293T cells - (reply: 2)
  79. Which epitope tag to use for ChIP? - (reply: 1)
  80. Total Protein in COS-7 Cells - (reply: 1)
  81. protein extraction from cell nucleus - (reply: 2)
  82. Determining phosphorylation status of a protein by transfection - (reply: 5)
  83. Need to strip the membran before probe the internal control protein - (reply: 3)
  84. IgG purification from hybridomas - (reply: 2)
  85. Low molecular weight proteins below 30kDa - (reply: 3)
  86. Is NuPAGE Bis-Tris gel for native or denaturing protein? - (reply: 1)
  87. Separation of glycerol from protein - (reply: 6)
  88. Is Protein A present on the Staphylococcus saprophyticus cell wall too? - (reply: 2)
  89. How does the first ribosomal protein get synthesized? - (reply: 2)
  90. Protein bandshift in western blot of cell lysate vs immunoprecipitation eluent - (reply: 4)
  91. transformation and protein purification calculation help; very basic - (reply: 5)
  92. relationship between protein size, lysate concentration, and band intensity - (reply: 3)
  93. cytoplasmic protein - (reply: 1)
  94. B Per for total bacterial protein extraction - (reply: 2)
  95. transfer of low molecular weight protein - (reply: 3)
  96. phospho-protein and total protein significance - (reply: 1)
  97. Sudden problems with Separating Proteins using SDS Gel - (reply: 4)
  98. Rate of protein production with pcDNA 3.3 transfected into HEK293 - (reply: 3)
  99. no protein expression - (reply: 3)
  100. software to predict domains to proteins interactions - (reply: 5)
  101. protein half-life assesment - (reply: 3)
  102. Do protein purification under partially denatured condition - (reply: 1)
  103. Discoloration of Nickel affinity resins during 6-his protein purification - (reply: 1)
  104. Protein precipitation by storage - (reply: 11)
  105. designing fusion protein with Flag tag - (reply: 1)
  106. Problems with biotynylated protein binding to monomeric avidin - (reply: 1)
  107. Control protein for eukaryotic expression - (reply: 5)
  108. Protein precipitation method - (reply: 2)
  109. Protein extracted from RNAlater tissues is blue... Help! - (reply: 2)
  110. Flag fusion protein not detected by Flag antibody - (reply: 8)
  111. Protein repeatedly does not express from pMX retroviral vector-advice needed des - (reply: 10)
  112. No reduction in protein expr after 2nd passage of transfected cells - (reply: 3)
  113. what are albumin associated proteins ?and can you please name them? - (reply: 1)
  114. Which domains are commonly found to mediate protein protein interaction? - (reply: 1)
  115. instability index of expressed protein - (reply: 1)
  116. Protein Purification-catastrophe while purifying? - (reply: 2)
  117. differentiating proteins in/on bacteria - (reply: 1)
  118. Protein purification for adherent mammalian cells - (reply: 2)
  119. Zero absorbance by Bradford assay for Dialyzed protein - (reply: 18)
  120. SDS and urea lysis, protein degradation and freeze-thaw cycles - (reply: 4)
  121. Prey protein band bleeds, IgG band normal? - (reply: 3)
  122. Phosphorylation of recombinant proteins in E. coli - (reply: 1)
  123. No proteins after HeLa cell lysis - (reply: 3)
  124. What is Role of HA in the biology of influenza virus and how this protein influe - (reply: 1)
  125. Malachite Green ATPase Assay - How to deactivate the protein and what is the sen - (reply: 1)
  126. Protein expression - which is more sensitive/accurate? - (reply: 1)
  127. half life of a protein - (reply: 3)
  128. Can protein isomers be detected in SDS-PAGE - (reply: 3)
  129. Blocking a protein in cell culture through antibody - (reply: 10)
  130. Luciferase fusion protein? - (reply: 1)
  131. NusA tag produces protein truncations? - (reply: 3)
  132. Probing protein - (reply: 1)
  133. Tissue-Specific Protein Expression Database - (reply: 4)
  134. can anyone help me with calculating a molar ratio of protein to lipid? - (reply: 4)
  135. Problem with strep tagged protein purification - (reply: 5)
  136. production of phosphorylated proteins in E.coli host system - (reply: 1)
  137. why i get double band for recombinant protein - (reply: 9)
  138. What is the reason of doing proteomics work? - (reply: 2)
  139. Protein quantification - (reply: 4)
  140. Protein extraction for 2D electrophoresis - (reply: 2)
  141. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
  142. Test for protein in lysate - (reply: 4)
  143. How to dissolve TCA/acetone precipitated proteins? - (reply: 1)
  144. protein purification in acanthamoeba lysate - (reply: 3)
  145. LMW proteins not transfer of gel to membrane - (reply: 1)
  146. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
  147. screening Protein Expression - (reply: 1)
  148. Insufficient Transfer of proteins through semi dry transfer - (reply: 6)
  149. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
  150. co-ip of endogenous and tagged proteins - (reply: 1)
  151. purify protein from native gel - (reply: 1)
  152. purifying proteins with repeats - (reply: 3)
  153. Protein purification from greenish supernatant of Pichia pastoris - (reply: 10)
  154. BCA assay - (reply: 1)
  155. Elusive protein refuses to be found in human serum with ELISA - (reply: 3)
  156. SDS-PAGE abnormalities in proteins near to front dye - (reply: 5)
  157. Membrane protein - (reply: 1)
  158. adding Tween80 to bacterial culture for protein purification ?! - (reply: 3)
  159. Protein sequence conservation - (reply: 2)
  160. MALDI MS resutls to identify proteins - (reply: 2)
  161. Wrong protein size on western blot - (reply: 10)
  162. Protein measurement of Laemmli samples - (reply: 2)
  163. Anyone has experience in using estrogen receptor (ER) fusion protein? - (reply: 1)
  164. Difficulty in Expression of Protein Having 73% GC contents in GST Tag Vectrors - (reply: 3)
  165. Is there a such thing that would allow me to generate 2 individual proteins, but - (reply: 5)
  166. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
  167. how to detect the modification like phosphorylation on one amino acid locus of o - (reply: 2)
  168. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
  169. Problem in acetone precipitation of plant proteins. - (reply: 1)
  170. Trouble with purified recombinant protein bands - (reply: 3)
  171. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
  172. Extraction of Transmembrane Proteins - (reply: 1)
  173. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
  174. Protein folding in different species - (reply: 1)
  175. Problems in expression of target protein in E. coli - (reply: 3)
  176. Protein structure modeling for enzymes with point mutations - (reply: 3)
  177. Membrane protein extraction - (reply: 2)
  178. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
  179. Protein activation after purification - (reply: 4)
  180. Protein amount to load for publication figure? - (reply: 4)
  181. Identifying new Ca2+-binding proteins - (reply: 7)
  182. Membrane Protein Extraction - (reply: 2)
  183. protein fractionation - (reply: 4)
  184. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
  185. What is different between Native protein and Denatured protein samples. - (reply: 4)
  186. Insoluble Protein of Interest Resulted from Mammalian Gene Expression - Can Any - (reply: 3)
  187. Isolation of protein and RNA from RNeasy Minikits? - (reply: 5)
  188. IgG heavy chain from IP - (reply: 8)
  189. protein extraction and peptide quantification from fat breast tissue - (reply: 2)
  190. experiments to determine the relationship between proteins - (reply: 4)
  191. trouble in his-tagged protein binding with Ni-NTA agarose - (reply: 3)
  192. Contaminants in protein purification.....help! - (reply: 3)
  193. Western Blotting loading control for large proteins - (reply: 2)
  194. Protein expression and purification - Please help - (reply: 2)
  195. Protein purifcation without chromatography - (reply: 6)
  196. Protein of 460 kD on western blot - (reply: 3)
  197. tag for protein purification - (reply: 7)
  198. Quantitation of protein concentration in rehydration buffer - (reply: 3)
  199. Protein is not Binding to DE52 column material - (reply: 2)
  200. Are two promoters needed for co-expressing 2 proteins from the same plasmid in S - (reply: 4)
  201. membrane proteomics - (reply: 1)
  202. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
  203. MBP-Tagged protein degrading after expression - (reply: 1)
  204. How to estimate the protein expression level from the SDS_PAGE? - (reply: 8)
  205. Protein quantification using HPLC - (reply: 1)
  206. Western Quantification (No BCA???) - (reply: 2)
  207. Protein extraction and isolectric focusing - (reply: 1)
  208. Western Blot: only non-specific bands and not protein of interest - (reply: 3)
  209. Inhibition of a protein upstream in signaling cascade ? - (reply: 5)
  210. fast human protein function catalog? - (reply: 3)
  211. Protein puriification using IMAC: flowthrough very slow - (reply: 2)
  212. Sediments/debris/pellet in protein lysate? - (reply: 3)
  213. Trouble in GST- fusion protein purification - (reply: 1)
  214. Helppurification of expressed protein - (reply: 2)
  215. Protein ladder not separating - (reply: 6)
  216. protein expression by changing start codon - (reply: 1)
  217. low concentration protein purification - (reply: 1)
  218. Western Blot - Protein Marker Present on Film After Developing - (reply: 1)
  219. Host cell protein sequence search - (reply: 1)
  220. (best cell lysis method for) protein expression analysis by western blot - (reply: 2)
  221. Using centrifugal filter units to concentrate protein - (reply: 10)
  222. Protein isolation and lack of sop's - (reply: 1)
  223. membrane proteins ultrafiltration - (reply: 2)
  224. IgG control Immunofluorescence : have problem - (reply: 9)
  225. Determining protein concentration after solubilisation in Triton X-100 - (reply: 2)
  226. Purification of RNA binding proteins by avidin agarose beads - (reply: 2)
  227. specific phosphorylation - (reply: 2)
  228. Production of GST tagged fusion proteins in BL21 Star cells - (reply: 2)
  229. Conversion of protein from kDa to gram/L - (reply: 4)
  230. Reference protein give different expression - (reply: 3)
  231. how to convert RFU/sec of my Vmax in mol/min/mg of protein?? - (reply: 4)
  232. recombinant protein form - (reply: 2)
  233. EMSA: cell extract vs purified protein - (reply: 2)
  234. Low Protein Content In Cell Lysate - (reply: 1)
  235. Membrane protein concentration - (reply: 1)
  236. get EST/ cDNA accession number for protein - (reply: 1)
  237. Western blot question- High % Gel/Low MW Protein - (reply: 7)
  238. Protein expression opposite of mRNA expression. Ideas? - (reply: 6)
  239. tagged protein size - (reply: 10)
  240. Protein concentration of a labelled membrane protein - (reply: 2)
  241. WB of cellular proteins in human serum - (reply: 2)
  242. Protein purification method to isolate elicitor with biological activity - (reply: 1)
  243. Protein expression in e.coli - (reply: 5)
  244. problem in extracting the protein - (reply: 1)
  245. purification and binding of protein in denaturing conditions - (reply: 2)
  246. BCA Protein Assay and ELISA afterwards - (reply: 1)
  247. crosslinking of protein and generate antibody - (reply: 2)
  248. Protein purification problems: Presence of contaminated proteins along with the - (reply: 5)
  249. protein sample floating out of the tricine gel - (reply: 3)
  250. minimum recombinant protein amounts for gst pulldown - (reply: 4)
  251. Protein Purification from Polyacrylamide Gels - (reply: 3)
  252. Protein Expression vector with small tag - (reply: 1)
  253. Protein precipitated while kept at 4C over night after elution from a Ni-NTA col - (reply: 4)
  254. Protocol for elution of desthiobiotin-labelled proteins from Streptavidin Column - (reply: 2)
  255. Software for scanning of specific aa sequence in proteins - (reply: 5)
  256. Membrane Protein SDS PAGE and WB - (reply: 1)
  257. Wet transfer of proteins between 100-200kDa - (reply: 2)
  258. Formula of crude protein analysis - (reply: 1)
  259. Large-Scale Purification of Non-Secreted Protein in E.Coli or CHO Cells - (reply: 7)
  260. protein concentration and Pull down - (reply: 3)
  261. High Yield Protein Expression 10g/L - (reply: 5)
  262. No activity of expressed protein obtained from pET32a cloned gene - (reply: 6)
  263. GST- tagged protein expressed in insoluble - (reply: 5)
  264. Purifying a "protein" - (reply: 2)
  265. Antibodies against protein complex - (reply: 4)
  266. converting protein names protein ids and enterz gene ids - (reply: 1)
  267. SDS solubilisation, Membrane Proteins and Micelle Formation - (reply: 2)
  268. Prepare protein standards for concentration determination - (reply: 1)
  269. Protein degradation on 2D gels - (reply: 2)
  270. E-book on recombinant protein expression - (reply: 1)
  271. small proteins - (reply: 2)
  272. protein Protein Linking - (reply: 5)
  273. Protein and RNA interaction - (reply: 1)
  274. SDS PAGE - Band artifacts when protein buffer is pH 5.0 and heated - (reply: 2)
  275. Immunoprecipitated protein visible on coomassie but not detected by Western - (reply: 4)
  276. dialyse of a protein - (reply: 4)
  277. Elimination of residual protein in cytosol - (reply: 2)
  278. Trouble estimating my protein size using ladder (image included) - (reply: 2)
  279. Bradford vs Lowry protein concentration gives dramatic different results - (reply: 5)
  280. Eliminating Protein/Protein Interactions for Western Blot - (reply: 2)
  281. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
  282. Inclusion body formation of the recombinant protein expressed in pET28a in E.col - (reply: 3)
  283. Is there any software that can predict DNA binding proteins based on input DNA s - (reply: 2)
  284. Tagged recombinant protein has different mobility? - (reply: 4)
  285. How to stabilized a recombinant protein - (reply: 1)
  286. Total protein determination before Elisa? - (reply: 2)
  287. getting negative protein measurements - (reply: 3)
  288. Mass-spectrometry of membrane proteins - (reply: 1)
  289. CCRK (CDK20) protein expression and western blot - (reply: 7)
  290. Human IgG Elisa variability problems - (reply: 2)
  291. My protein doesn't bind to the Ni-NTA column - (reply: 11)
  292. Phosphotidyl ethanolamine conjugated protein - (reply: 1)
  293. TFE in inducing alpha-helix formation in proteins - (reply: 3)
  294. Quantify Chromatin With Protein Assay? - (reply: 1)
  295. Acetone Precipitation of proteins - (reply: 1)
  296. Mouse IgG detection - Blocking ? - (reply: 2)
  297. TCA IgG precipitation - (reply: 2)
  298. How to load equal amount of protein without using spectramax - (reply: 4)
  299. metal chelation for proteins - (reply: 3)
  300. protein not stained by Coomassie Blue - (reply: 13)
  301. Protein stability at pH8.3 - (reply: 2)
  302. No resolving of protein/bands below 50kDa :-s - (reply: 5)
  303. Designing truncated protein as antigen - (reply: 2)
  304. protein is not expessing in HEK 293 cells - (reply: 2)
  305. Transferring >150 kDA proteins conditions - (reply: 3)
  306. Purification of FC tagged proteins - (reply: 3)
  307. Brief vortexing for reconstitution of recombinant proteins? - (reply: 1)
  308. question about protein expression normalization. - (reply: 6)
  309. protein induction from yeast - (reply: 1)
  310. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  311. In vitro transcription/translated protein to cells!!! - (reply: 1)
  312. Expressing protein in non-E.coli - (reply: 2)
  313. Protein bands not migrating - (reply: 3)
  314. Tumor protein precipitation at denaturing step? - (reply: 1)
  315. Does triple labeled protein precipitate more easily? - (reply: 2)
  316. diluting standards BCA assay - (reply: 2)
  317. measurement of proteins in a mixture of soluble-insoluble proteins - (reply: 5)
  318. Role of Isoleucine in protein structure. - (reply: 1)
  319. Cell confluency, transfection, protein expression for 293T cells - (reply: 2)
  320. Large proteins in Western - (reply: 12)
  321. SDS-PAGE trouble with protein concentration - (reply: 2)
  322. Protein Size 50 kDa higher than expected - (reply: 3)
  323. protein extraction from human tissue (very small) to do ELISA - (reply: 5)
  324. Protein precipitation from RIPA buffer to perform ELISA - (reply: 4)
  325. Method for protein concentration measurement - (reply: 8)
  326. Stable protein expression in Vero cells - (reply: 2)
  327. Why GFP gets cutted from my fusion protein? - (reply: 9)
  328. Phospho-protein vs total protein Western blot - (reply: 5)
  329. Relationship between total protein and enzyme activity - (reply: 3)
  330. protein with good soulbility but doesnt bind to Ni-NTA coloumn - (reply: 4)
  331. GST fusion protein purification - (reply: 2)
  332. Is protein degrade by UV ? - (reply: 4)
  333. Can I test protein denaturation after sonication - (reply: 2)
  334. myc-tagged proteins - (reply: 1)
  335. GST-tagged protein expression problem! :-( - (reply: 4)
  336. Uniprot protein corresponding nucleotide sequence - (reply: 1)
  337. Co-IP: Prey Protein Present in Neg and Pos Eluates - (reply: 5)
  338. How OD600 influence on IPTG induction and protein expression - (reply: 5)
  339. Protein extraction from mouse tissue? - (reply: 1)
  340. Can a anti-IgM antibody developed for BCR bind IgM - (reply: 1)
  341. finding the protein and nucleotide matches - (reply: 1)
  342. Mouse IgG1 purification - Protein G - (reply: 1)
  343. Overexpressed protein shows a band higher than expected?! - (reply: 2)
  344. Why can't we detect multiple protein in a single membrane using western blot - (reply: 2)
  345. Bradford Protein Assay - Problem in BSA Standard Curve - (reply: 2)
  346. Protein structure prediction - (reply: 1)
  347. sedimentation of cytosolic proteins - (reply: 2)
  348. Determining pI of a protein - (reply: 3)
  349. Multiple protein bands after expression - (reply: 6)
  350. ethanol precipitation of protein - (reply: 1)
  351. Could anyone help me out with the removal GST tag from my recombinant protein? - (reply: 1)
  352. co-eluted protein with his-tag protein - (reply: 1)
  353. Help with His-Tag protein purification - (reply: 2)
  354. Use dialysis tube to concentrate virus - (reply: 8)
  355. How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
  356. protein showing up around 50 kda rather than 20 kda - (reply: 2)
  357. Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
  358. Is DTT reduction and alkylation critical before concentrating proteins? - (reply: 1)
  359. cloning without phosphorylation - (reply: 7)
  360. Concentrating a diluted protein and protein determination - (reply: 4)
  361. IHC: confirming specificity phosphorylated protein antibody - (reply: 2)
  362. protein extraction from GEL - (reply: 5)
  363. Decreased protein yields after successive passaging? - (reply: 1)
  364. Constitutive p42/44 MAPK phosphorylation - (reply: 2)
  365. His Tag Cleavage (protein precipitates) - (reply: 3)
  366. protein biochemistry - (reply: 1)
  367. How to distinguis soluble from insoluble recombinant proteins produced in E. col - (reply: 1)
  368. fusion of cDNA expressing protein with gfp - (reply: 1)
  369. GFP fusion protein not expressing! - (reply: 2)
  370. can Protamine sulfate inhibit or degrage a protein? - (reply: 1)
  371. plant extraction procedure for protein - (reply: 3)
  372. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
  373. Transfer of proteins from gel to PVDF for short period - (reply: 3)
  374. interest protein and total protein ratio range - (reply: 2)
  375. Can not reduce protein level - (reply: 1)
  376. Precipitation during dialysis - (reply: 2)
  377. Cloning genetic sequences of proteins. - (reply: 1)
  378. Experience with Y-PER Plus, dialyzable yeast protein extraction reagent - Pierce - (reply: 1)
  379. Fold-changes different when quantifying same protein via IF and Western - (reply: 1)
  380. Low expression levels of highly soluble protein - why is ON expression not helpi - (reply: 8)
  381. target mRNA/protein levels increase with siRNA - (reply: 2)
  382. How exactly do i show two proteins interacting? - (reply: 2)
  383. Reversed-Phase chromatography for protein purification - (reply: 3)
  384. All-encompassing proteomics workflow? - (reply: 1)
  385. Western Blot Antibody Epitope - (reply: 1)
  386. Easy method to lyse E. coli pellet (follow-up of GST fusion protein purification - (reply: 4)
  387. problem with purification of gst-tagged protein - (reply: 2)
  388. protein aggregation (?) in gelatin zymography - (reply: 1)
  389. Signal peptide removed when making recombinant protein - (reply: 1)
  390. problem with protein identification - (reply: 8)
  391. Methods of concentrating total protein extracts - (reply: 2)
  392. All mRNA translated into protein - (reply: 2)
  393. Protein degradation during expression - (reply: 3)
  394. Protein extraction - (reply: 5)
  395. Precipitation Problems with Concentrating Proteins with PEG - (reply: 1)
  396. Commercial standard protein ladder/marker >250? - (reply: 2)
  397. protein size determining methods - (reply: 1)
  398. Proteins below 60 kDa not resolving in SDS-PAGE!! - (reply: 1)
  399. Immunofluorescence for detecting three proteins. - (reply: 2)
  400. Best gel percentage to separate 24 and 36 kDa proteins - (reply: 3)
  401. Can carbamylation of proteins take place without heating? - (reply: 1)
  402. How to rescue my proteins? - (reply: 2)
  403. Expression of insoluble protein - (reply: 4)
  404. How to get pure protein of a gene - (reply: 1)
  405. Homogenisation of protein - (reply: 1)
  406. How to get soluble protein? - (reply: 3)
  407. protein concentration/well calculation - (reply: 1)
  408. How to get pure protein of a gene - (reply: 3)
  409. study of the role of a protein in a lesion - (reply: 3)
  410. IPTG induced protein go to inclusion bodies - (reply: 1)
  411. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
  412. Protein Isolation from mouse brain: problems - (reply: 2)
  413. Protein extraction from dead cells - (reply: 1)
  414. Problems with high molecular weight protein transfer in western blot - (reply: 1)
  415. Expression level analysis at mRNA vs protein level - (reply: 6)
  416. Trouble Western Blotting a 360kDa Protein - (reply: 5)
  417. Purifying recombinant protein from pellet? - (reply: 2)
  418. Protein ladder - (reply: 1)
  419. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
  420. Question about the amount of mRNA and secreted protein level!!! THX~ - (reply: 1)
  421. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  422. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
  423. membrane protein expression - (reply: 1)
  424. normalization to protein content or cell number - (reply: 2)
  425. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
  426. Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 6)
  427. Magnesium acetate in protein lysates - (reply: 1)
  428. detection of phosphorylation from cell pellet - (reply: 2)
  429. How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? - (reply: 6)
  430. Protein sequencing gaps - (reply: 1)
  431. which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
  432. Suggestions for experimental or in silico validation related to ncRNA or protein - (reply: 1)
  433. desired protein is going in pellet...do not want to go for renaturation - (reply: 3)
  434. Concentrating protein of a particular size - (reply: 1)
  435. Extra bands after protein expression/purification - (reply: 4)
  436. 96 well protein expression - (reply: 1)
  437. Different cell lines different protein stability - (reply: 1)
  438. Better protein Expression? - (reply: 2)
  439. Anti-human IgG phosphatase give negative results - (reply: 13)
  440. protein extraction from transwell inserts - (reply: 2)
  441. Expression of protein with pI 8.2 - (reply: 1)
  442. Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
  443. Methionine for protein expression - (reply: 3)
  444. Protein expression in yeast - Yeast Genotype - (reply: 1)
  445. Carrier protein concentration - (reply: 2)
  446. protein - (reply: 1)
  447. Anti-his antibodies - Noob to protein work - (reply: 10)
  448. Protein localization studies - (reply: 5)
  449. Protein Expression problems - (reply: 4)
  450. Cell Envelope Proteins Smeared - (reply: 1)
  451. Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection - (reply: 2)
  452. Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
  453. predict possible protein protein interaction - (reply: 1)
  454. Cellular localisation of a protein - (reply: 1)
  455. Sequential phosphorylation in vivo but not in vitro - (reply: 4)
  456. problem in expressing A DNA binding protein - (reply: 6)
  457. Detection of glycosylations on Western blotted proteins - (reply: 2)
  458. Problems with protein estimation and western blots - (reply: 1)
  459. histone proteins extraction and 2D - (reply: 1)
  460. Quantity of protein to charge - (reply: 1)
  461. Determining the N- and C-terminal regions in a protein - (reply: 6)
  462. Properties of amino acids and protein functional activity - (reply: 1)
  463. Phosphorylation - (reply: 2)
  464. Protein tagging with GFP/effect of expression construct - (reply: 2)
  465. RNAi problem - Gene is knocked down, but not protein - (reply: 8)
  466. Insoluble Protein purification - (reply: 1)
  467. Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
  468. my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
  469. Missing expression of proteins of interest in Pichia pastoris - (reply: 2)
  470. BLASTing protein block - (reply: 1)
  471. IgG control - (reply: 6)
  472. Difficulty in expressing fusion protein - (reply: 1)
  473. IgG control - (reply: 4)
  474. Native PAGE, interaction of proteins, validity - (reply: 4)
  475. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
  476. Peptide Protein Conjugation - (reply: 2)
  477. Same non specific band for different target proteins - (reply: 1)
  478. Types of 96-well plates used for BCA protein quantification - (reply: 1)
  479. Protein measurements in 2% Triton X samples - (reply: 2)
  480. No protein in cytoplasmic extract - (reply: 1)
  481. Size and composition of spacer between protein and tag? - (reply: 4)
  482. Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
  483. protein extraction from pirmary keratinocytes - (reply: 2)
  484. his tagged protein - (reply: 1)
  485. How to purify unstable protein? - (reply: 10)
  486. unstable flag tagged protein - (reply: 4)
  487. Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
  488. SDS-PAGE of negatively charged protein - (reply: 4)
  489. GFP tagged protein expression detection - (reply: 1)
  490. How to obtain high-level production of low molecular weight recombinant proteins - (reply: 1)
  491. How to quantify the protein amount for serum samples ? - (reply: 1)
  492. Insoluble Protein - (reply: 5)
  493. making recombinant protein - (reply: 2)
  494. Problem with expression of recombinant protein - (reply: 3)
  495. how to make a recombinant protein with signal peptide - (reply: 1)
  496. Large protein blot ~400kDa - (reply: 3)
  497. Protein folding affected by neg. cahrges amino acids? - (reply: 4)
  498. getting rid of imidazole in purified protein? - (reply: 2)
  499. which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
  500. Western Blotting of Proteins less than 10 kDa - (reply: 1)
  501. Protein wight in kDa - (reply: 2)
  502. BN-PAGE of purified membrane associated protein - (reply: 5)
  503. Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
  504. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  505. Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
  506. Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
  507. My protein samples are running abnormally on a SDS PAGE - (reply: 3)
  508. Fusing permanently a phosphate molecule to a protein. - (reply: 4)
  509. Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
  510. Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
  511. Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
  512. ChIP-PCR: Amplification in IgG negative control - (reply: 1)
  513. Precipitations in protein dialysis - (reply: 1)
  514. mRNA increase and protein expression - (reply: 10)
  515. large protein - (reply: 3)
  516. applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
  517. A problem with IgG purification by Protein G chromatography - (reply: 4)
  518. problem with loading the protein on to SDS-Gel - (reply: 1)
  519. anti-GLUT4 antibody, extracellular epitope? - (reply: 1)
  520. Protein contamination with DNA - (reply: 5)
  521. Western blot not showing protein - (reply: 2)
  522. Western Blot not showing protein - (reply: 4)
  523. No activity of fungi protein in E.coli - (reply: 15)
  524. longer running of proteins in SDS PAGE! - (reply: 2)
  525. Rehydrating/Resuspending Drosophila Proteins - (reply: 1)
  526. How exactly does an IgG antibody control in ChIP work? - (reply: 3)
  527. PacI affect protein translation? - (reply: 1)
  528. ELISA Kits for proteins in brain homogenates - (reply: 6)
  529. Do we really need to do protein quantification - (reply: 4)
  530. Maximum number of proteins - (reply: 1)
  531. protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
  532. Western Blot for 320KD Protein - (reply: 4)
  533. Can someone recommend a good proteomics company/facility with Orbitrap and can p - (reply: 1)
  534. Dot Blot - Protein not adhering to membrane - (reply: 1)
  535. Smears after protein precipitation - (reply: 4)
  536. protein expression level and OD600 in E.coli - (reply: 1)
  537. Protein contamination - How to remove all protein from my DNA? - (reply: 3)
  538. FACS Sorting and Protein Purification - (reply: 5)
  539. How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
  540. biotinylated protein extracts and antigen-antibody stringency - (reply: 3)
  541. overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
  542. Protein purification buffer - (reply: 3)
  543. Protein purification in native conditions - (reply: 1)
  544. Membrane proteins - (reply: 6)
  545. Protein extraction efficiency - (reply: 2)
  546. Triton X-100 and Dialysis - (reply: 2)
  547. homology proteins - (reply: 1)
  548. Improving expression of recomb proteins in baculovirus - (reply: 1)
  549. Inhibition of Protein Synthesis in Eukaryotes - (reply: 1)
  550. Trizol and Protein extraction - (reply: 2)
  551. Protein Extraction SDS-Buffer Pellet not Dissolving and Laemlli Bufffer Green? - (reply: 3)
  552. Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
  553. Western Blot Two Proteins - (reply: 1)
  554. Western Blot Protein Length - (reply: 4)
  555. Increase toxic protein expression? - (reply: 1)
  556. Recombinant protein from cell lysate - (reply: 4)
  557. Gooey DNA is protein samples after adding sample buffer - (reply: 1)
  558. pET47b proteins not expressing in BL21 (DE3) - (reply: 5)
  559. Thrombin removel His tag protein digestion - (reply: 1)
  560. protein expression vector.. - (reply: 4)
  561. ChIP-Seq with HA-tagged protein - (reply: 1)
  562. Fc fusion protein smearing on SDS PAGE - (reply: 2)
  563. protein solution cloudy after dialysis - (reply: 5)
  564. Using protein content to standardize ELISA data? - (reply: 2)
  565. How to improve protein solubility in E. coli? - (reply: 11)
  566. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
  567. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
  568. type of IgG in my sample?? - proteinA purified sample (reply: 1)
  569. To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
  570. Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
  571. Protein structure prediction - (reply: 2)
  572. Help with small protein with his-tag purification - (reply: 7)
  573. protein precipitate at pI?? - (reply: 1)
  574. Protein agregation in WB samples - (reply: 3)
  575. Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
  576. Protein dissolved - After acetone precipitation (reply: 2)
  577. Max amount of protein - (reply: 4)
  578. TCA/Acetone protein precipitation - (reply: 9)
  579. how to add 3XHA to the N-terminal of a protein - (reply: 5)
  580. Separating protein isoforms and quantifying activity - (reply: 3)
  581. Protein refolding - (reply: 2)
  582. Freezing proteins at -80 - (reply: 1)
  583. Overrepresentation of genomic region in ChIP IgG control?! - (reply: 2)
  584. Discrimination against denatured proteins?! - Can ELISA discriminate agains denatured proteins? (reply: 2)
  585. Chromatin Protein IP - (reply: 2)
  586. How can I detect mannosidase activity of a protein - (reply: 1)
  587. Protein Aggregation with 6xHis tagged CreGFP - (reply: 1)
  588. Problem with protein resolving in electrophoresis - (reply: 7)
  589. Protein stability at room temperature - (reply: 3)
  590. Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
  591. protein pass through column - (reply: 1)
  592. Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
  593. protein precipitation - isolation of proteins (reply: 1)
  594. effect of lysozyme on gst-fusion protein - (reply: 1)
  595. Protein purification from HEK 293 cells - (reply: 1)
  596. Odd result help - Luciferase / protein / mRNA levels (reply: 1)
  597. Upscaling protein expression in E.coli - (reply: 2)
  598. Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
  599. confirm proteins interacting with histones using acid extraction - (reply: 4)
  600. Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
  601. nuclease contamination in protein prep - (reply: 4)
  602. Bio Rad protein assay for liver homogenate - (reply: 1)
  603. protein purification - (reply: 1)
  604. Bradford Vs beta-actin for WB protein quantification - (reply: 3)
  605. Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
  606. SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
  607. acrylamide gel for low molecular weight protein - (reply: 2)
  608. ELISA kits protocols, antibodies, control proteins, and more - (reply: 1)
  609. HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
  610. Pierce BCA Assay problem - Protein values bizarre (reply: 3)
  611. plant protein purification opinion - (reply: 3)
  612. WB - Transferring REALLY big proteins - (reply: 2)
  613. Unable to Purify protein! - Protein purification (reply: 2)
  614. Cloning and expressing a mystery 71KDa protein - (reply: 9)
  615. HIS-tagged protein purification - (reply: 12)
  616. how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
  617. Is it possible to islate strong negative charged protein - (reply: 1)
  618. Protein expression vectors Please help - (reply: 2)
  619. what is protein flexibility - (reply: 1)
  620. His-tagged protein purification problem - (reply: 2)
  621. protein level expression - (reply: 1)
  622. How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
  623. Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
  624. proteins, new ones? - How to know (reply: 8)
  625. KO mice show band for knocked-out protein - (reply: 1)
  626. Trouble in protein quantification - (reply: 2)
  627. SDS-PAGE gel wont separate small proteins - (reply: 5)
  628. LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
  629. Unexpected expression of my protein in HepG2 - (reply: 3)
  630. Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
  631. EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
  632. Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
  633. GST-fusion protein degrades in-vivo - (reply: 3)
  634. Purification of recombinant proteins from insect cells - (reply: 2)
  635. Non reducing protein ladder? - (reply: 5)
  636. similar size of proteins but different movement on SDS-PAGE - (reply: 1)
  637. Protein Expression in yeast - protein not expressed (reply: 1)
  638. SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
  639. no detection of proteins at 25KDa - (reply: 4)
  640. help with protein elution - (reply: 1)
  641. Protein quantification after 8M urea lysis - (reply: 3)
  642. phosphorylted protein smaller not bigger? - (reply: 1)
  643. protein phosphorylation status in sample buffer - (reply: 2)
  644. Protein detection - western blot (reply: 3)
  645. Boiling, protein aggregation......reversal?? (western) - (reply: 3)
  646. expression of protein - (reply: 1)
  647. Tag proteins for CO-IP and confocal - (reply: 1)
  648. insoluble protein expression help - (reply: 3)
  649. Active protein extract from corn - (reply: 2)
  650. Model Protein - Need Advice! - (reply: 3)
  651. You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
  652. CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
  653. His-tag protein vs. BSA - (reply: 3)
  654. Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
  655. YFP protein fusion accumulates - (reply: 10)
  656. Protein Concentation from M to mg - (reply: 2)
  657. Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
  658. Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
  659. protein interactions - (reply: 2)
  660. MTT assay, protein assay analysis - Need assistance with analysis of these two assays (reply: 6)
  661. Lactobacillus crude protein extraction? - (reply: 8)
  662. Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
  663. Western Blot of Large Protein Help - (reply: 1)
  664. Fusion tagged protein and Western Blot problem - (reply: 3)
  665. Compatible detergents for proteomics - (reply: 4)
  666. Troubleshooting Protein Purification using FPLC - (reply: 1)
  667. how to make protein soluble? - protein solubility (reply: 4)
  668. Quantification of proteins in Western Bands - (reply: 2)
  669. protein yield is very low after purification - protein purification (reply: 9)
  670. his- & biotin- tag protein purfication - (reply: 2)
  671. How to remove protein to show an activity is protein based - (reply: 7)
  672. FZR1 PROTEIN EXPRESSION - (reply: 1)
  673. help with his tagged protein purification - precipitation problem (reply: 1)
  674. Overexpression plasmid is not expressing protein - (reply: 13)
  675. Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
  676. why phosphorylated protein relative to total protein? - (reply: 2)
  677. SDS-PAGE with 500 ug protein - (reply: 3)
  678. Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
  679. His tag protein purification - (reply: 7)
  680. Dry protein gels without cracking - (reply: 5)
  681. Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
  682. insoluble fusion protein - (reply: 1)
  683. Protein precipitating need help choosing suitable buffering system - (reply: 3)
  684. Total Cell protein - (reply: 1)
  685. ammonium precipitation of Proteins - (reply: 2)
  686. Expressing human protein in E.Coli - (reply: 12)
  687. Assay in Blood - sticky protein (reply: 13)
  688. dye protein ladder - (reply: 5)
  689. protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
  690. Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 7)
  691. Naming system of IgG and Identification of IgG subclass - (reply: 5)
  692. How do you identify protein detected on western blot from a lysate???? - (reply: 2)
  693. how to express protein in soluble form - (reply: 1)
  694. Help with GST protein purification - (reply: 4)
  695. Antiobiotics for protein expression - (reply: 3)
  696. Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
  697. IP no target protein in elution - (reply: 1)
  698. protein function prediction - (reply: 5)
  699. stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
  700. Protein stability - (reply: 4)
  701. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
  702. Protein isolation in zebrafish - (reply: 1)
  703. how to extract proteins from HEK293 - I need your experience (reply: 3)
  704. Identification of purity of the protein - (reply: 2)
  705. ChIP - protein contamination of ChIP'ed material - (reply: 4)
  706. Biotinylation of protein in tris buffer - (reply: 2)
  707. Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
  708. HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
  709. Protein kinase protocols - (reply: 2)
  710. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
  711. Purification of His tagged protein - (reply: 2)
  712. BCA diluent and standard curve - (reply: 1)
  713. protein induction problem - (reply: 4)
  714. Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
  715. Protein purification. How to make function of recombinant protein? - (reply: 2)
  716. Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
  717. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
  718. ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
  719. Protein expression declines after overnight incubation - (reply: 4)
  720. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
  721. elimination of Protein interference from DNA - for RT-PCR (reply: 2)
  722. Fusion protein linker - (reply: 2)
  723. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
  724. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
  725. Protein Phosphorylation - (reply: 2)
  726. Western for very small protein - (reply: 3)
  727. Protein streaking on Blue Native-PAGE - (reply: 9)
  728. basic questions about protein assays and cuvettes - (reply: 4)
  729. His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
  730. antibody production-how to differentiate epitope as foreign - (reply: 4)
  731. How to attain pure chains of one protein complex - (reply: 1)
  732. MINI Dialysis Units - below 2K MWCO? (reply: 1)
  733. Help for co-transfection of proteins and plasmid - (reply: 1)
  734. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
  735. Fusion Protein - (reply: 3)
  736. Solubility of proteins - (reply: 1)
  737. Loss of protein upon concentration - (reply: 4)
  738. protein overexpression in HeLa cells - (reply: 1)
  739. His-tag protein purification - aggregation of protein (reply: 5)
  740. After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
  741. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
  742. High mRNA but no protein expression - (reply: 10)
  743. Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
  744. Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
  745. Decrease in concentration after protein purification - (reply: 3)
  746. Circular Dichroism and disordered proteins - (reply: 1)
  747. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
  748. Bacterial Induction: Same concentration of protein samples? - (reply: 1)
  749. protein phosphorylation - methods for protein phosphorylation (reply: 9)
  750. Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
  751. labeling a protein - (reply: 3)
  752. Co-IP of secreted proteins - (reply: 1)
  753. protein protein interaction discovery - (reply: 1)
  754. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
  755. Problem in stable expression of target protein using lentiviral vector - (reply: 3)
  756. How to be good at prep? - Calling all protein experts (reply: 2)
  757. Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
  758. His-tagged protein purification - (reply: 3)
  759. 143 kDa protein - (reply: 3)
  760. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
  761. protein purification - (reply: 3)
  762. Purification od insoluble protein (TIMP3) - (reply: 2)
  763. eluting gst fusion proteins - (reply: 1)
  764. How to pull down chromatin and identify protein associated with it? - (reply: 1)
  765. contamination in protein expression - I got a couple of questions (reply: 3)
  766. Protein extraction from perfusion fixed brain tissue - (reply: 5)
  767. Always expressing truncated protein - (reply: 3)
  768. Antibody production. - Discrimination between almost identical proteins. (reply: 1)
  769. secreted protein quantification - (reply: 2)
  770. protein sample degradation - (reply: 7)
  771. Major Protein Loss! Why? - (reply: 2)
  772. Protein in the nucleus vs in the cytoplasm - (reply: 1)
  773. Over Experssion of A Protein - (reply: 3)
  774. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
  775. problem in protein purification - (reply: 5)
  776. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
  777. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
  778. protein purification - (reply: 5)
  779. Cloning the coding region of a protein - (reply: 4)
  780. Problem with protein purification By FPLC - (reply: 4)
  781. Molar ratios of IgM and IgG antibodies in in vitro assays - (reply: 3)
  782. cytoplasmic and nuclear extraction for BCA - (reply: 6)
  783. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
  784. EGFP protein location problem fixing with PFA - (reply: 3)
  785. Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
  786. protein prep - about prptein preperation in diagnosis (reply: 1)
  787. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
  788. loss of protein during concentration - (reply: 6)
  789. protein degradation - (reply: 3)
  790. Cell lysates - Protein precipitation? - (reply: 3)
  791. Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
  792. His-tag proteins not eluted from Ni-Beads - (reply: 2)
  793. Is CNBr activated Sepharose good for binding IgG? - (reply: 3)
  794. Western Blots on Membrane Proteins - (reply: 9)
  795. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
  796. quantification of a protein in tissue - (reply: 3)
  797. protein sequencing company - (reply: 2)
  798. Protein extraction from mouse prostate - (reply: 3)
  799. Help - proteins faster than the dye front - (reply: 8)
  800. fluorescent proteins expressed in nucleus - (reply: 6)
  801. IGg or F(ab´)2 Fragment of IgG - HRP-conjugated secondary antibody (reply: 1)
  802. A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
  803. Using FASTA Seq to Compare protein Seq. - (reply: 1)
  804. Protein SDS-PAGE thick bands / smear - (reply: 4)
  805. Denaturing protein before blotting - (reply: 1)
  806. not completed transfer proteins on NT membrane - western blot (reply: 2)
  807. trouble with protein expression of high AT rich gene - (reply: 1)
  808. No protein lane have bands on Coomassie and western blot?! - (reply: 4)
  809. Protein cleavage and neurodegeneration. - (reply: 1)
  810. Extraction protein from paraffin-embedded tissues - (reply: 1)
  811. column chromatography - elution buffer for proteins (reply: 2)
  812. Lost protein after gel filtration - Gel Filtration (reply: 6)
  813. protein size - (reply: 1)
  814. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
  815. New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
  816. Weird effect of siRNA on protein in cells - (reply: 3)
  817. estimate size of protein - (reply: 2)
  818. Protein elution from GST-fusion protein complex - (reply: 3)
  819. Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
  820. total cell protein vs membrane protein - (reply: 4)
  821. amount of plasmid for transformation and fusion protein expression - (reply: 1)
  822. gst fusion protein - (reply: 3)
  823. Biological process from protein list - (reply: 3)
  824. how to get pure protein - (reply: 1)
  825. Liposome + IgG sample in SDS-PAGE - (reply: 1)
  826. Different centrifugation duration, different protein?? - (reply: 1)
  827. Protein tags - (reply: 6)
  828. What portion of protein can I get under this protocol? - (reply: 3)
  829. SDS-PAGE for proteins which have pI>7 - (reply: 1)
  830. looking for kinase inhibitor to study phosphatase - kinase inhibitor (Inhibit phosphorylation on transmembrane receptor) (reply: 4)
  831. to get protein in cytosol from inclusion bodies - (reply: 4)
  832. How can I get protein in soluble fration - (reply: 2)
  833. Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
  834. How long does it takes to make a crystallography of a protein? - (reply: 1)
  835. how to detect a knowen protein's receptor? - (reply: 1)
  836. Is IgG monoclonal antibody better then IgM? why? - why everyone prefer IgG? (reply: 3)
  837. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
  838. How much protein from a confluent 6 well plate? - (reply: 5)
  839. Protein expression - (reply: 8)
  840. Protein quantification - (reply: 2)
  841. immunochemistry - using cofocal to identify two protein localization (reply: 1)
  842. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
  843. Wet Transfer is not transferring 300kDa protein, why? - problems with wet transfer (reply: 11)
  844. Bands are lower than actual protein - (reply: 1)
  845. Protein lysate storage - (reply: 2)
  846. to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
  847. Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
  848. Protein Purification by precipitation - (reply: 2)
  849. refolding proteins using dialysis - (reply: 1)
  850. protein refolding - (reply: 1)
  851. concentrating protein by method other than tff - (reply: 1)
  852. Protein TAG for affinity purification - (reply: 1)
  853. BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
  854. Quantitating IP'd proteins - (reply: 1)
  855. help with protein localization study using GFP - (reply: 5)
  856. Protein shows KD but RNA level is higher - (reply: 4)
  857. No protein spots in 2D gel - Silver staining! - (reply: 17)
  858. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
  859. Wound healing (scratch) assay - extracellular matrix protein substrate (reply: 1)
  860. protein crystallography services question - (reply: 1)
  861. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
  862. Problem in expression of protein cloned in pcDNA3 - (reply: 1)
  863. Recombinant protein aggregation after purification - (reply: 2)
  864. average total protein content per 293t cell - (reply: 2)
  865. best and simple method to purify protein - (reply: 38)
  866. Protein A Sandwich ELISA - Protein A orients Capture Ab-Antigen-primary Ab-detector Ab (reply: 1)
  867. Localization of kanamycin resistance protein - (reply: 1)
  868. Are proteases a problem in protein quantification assays? - (reply: 3)
  869. why my protein precipitates? - (reply: 2)
  870. serum housekeeping proteins for total protein normalization - (reply: 1)
  871. serum housekeeping proteins for total protein normalization - (reply: 1)
  872. protein concentration per well - (reply: 6)
  873. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
  874. Detection of phosphorylation site - (reply: 23)
  875. help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
  876. Sites for protein sequence from DNA sequence - (reply: 2)
  877. will high-speed centrifuge rupture protein G? - (reply: 1)
  878. His-tag protein expression - (reply: 1)
  879. Does Change pH could affect protein interaction - GST pul down (reply: 2)
  880. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
  881. protein extraction - ripa buffer vs urea buffer (reply: 1)
  882. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
  883. problem about fused protein - problem about fused protein (reply: 2)
  884. Diffused protein in IF stainning - (reply: 2)
  885. IP my protein from culture supernatant - (reply: 2)
  886. How to concentrate the protein - (reply: 4)
  887. Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
  888. High pI proteins - High pI proteins (reply: 4)
  889. protein overexpression - factors affecting overexpression (reply: 3)
  890. NUCLEAR PROTEIN PREPARATION - (reply: 1)
  891. High Gene low protein levels - (reply: 1)
  892. Lost protein from blot? - (reply: 1)
  893. 293 vs 293TN cells - Which would be beter control in testing against expressed proteins (reply: 1)
  894. Protein extraction buffer compatible with 2D PAGE - (reply: 4)
  895. Protein Degradation after overexpression - (reply: 1)
  896. Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
  897. e coli strain selection for protein expression - (reply: 2)
  898. phosphorylation of primers using PNK - (reply: 1)
  899. IgG SDS-PAGE - (reply: 1)
  900. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
  901. wet or semi-dry transfer for low MW proteins - (reply: 4)
  902. Problems in upscaling protein expression - (reply: 2)
  903. Where is the future of the proteomics? - (reply: 5)
  904. problems with His tagged protein western blots - (reply: 3)
  905. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
  906. Protein aggregates SDS-PAGE - (reply: 5)
  907. check of amount of proteins on filter after rouge ponceau| - (reply: 1)
  908. dna loading dye for proteins - (reply: 2)
  909. too many isoforms expressing proteins - (reply: 2)
  910. protein optimization - (reply: 3)
  911. Protein stabilization buffer needed - (reply: 1)
  912. Freezing eluted His tag protein - (reply: 5)
  913. molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
  914. Protein Markers - (reply: 1)
  915. What if my expressed protein is toxic for the cells... - (reply: 1)
  916. His-tag protein lost it's tag - (reply: 2)
  917. AnionExchange problem: proteins do not bind - (reply: 12)
  918. Dialysis into Lipid - (reply: 2)
  919. How can I isolate ECM protein from a 6 well dish? - (reply: 4)
  920. How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
  921. can someone list some proteasome inhibitors? - co-IP for ubiquitiantion protein (reply: 4)
  922. GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
  923. Protein standard curves - (reply: 1)
  924. Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
  925. Western Blot for low expressed protein - (reply: 4)
  926. radiolabeled protein uptake - (reply: 2)
  927. small proteins have run ahead of tracking dye - (reply: 1)
  928. Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
  929. Precipitation of protein - (reply: 3)
  930. in vitro phosphorylation - (reply: 5)
  931. Meaning of Recombinant Protein - (reply: 3)
  932. Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
  933. Equal Protein Quantity - Loading onto Well (reply: 6)
  934. Desperate! Loss of basic proteins on 2D gels - (reply: 5)
  935. additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
  936. protein absorbance at 230nm??? - (reply: 1)
  937. How to look at protein stability? - (reply: 3)
  938. PEG Precipitation of IgM Antibodies - (reply: 2)
  939. Fermentas prestained protein marker issue! - (reply: 7)
  940. reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
  941. Expression of fusion proteins - (reply: 1)
  942. Help with protein extraction buffer problem!? - (reply: 1)
  943. Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
  944. Purification of Protein Extract for ELISA - (reply: 1)
  945. Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
  946. High protease activity in protein extraction - A problem in western blot (reply: 2)
  947. Number of proteins expressed in cell - (reply: 2)
  948. Deep sequencing to find gene targets of protein? - (reply: 1)
  949. Protein at low PH - Strange behavior of His-tagged protein at low PH (reply: 1)
  950. How to store wet dialysis membranes once opened? - (reply: 4)
  951. bands of protein seems like it did not move - (reply: 2)
  952. Constitutive association between two proteins - (reply: 2)
  953. Elute immunoprecipitated complex with protein G? - (reply: 1)
  954. protein indentification - protein to be identified on sds page based on MW (reply: 2)
  955. Help...purify and concentrate protein for IEF - (reply: 1)
  956. Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
  957. Could a 30aa peptides(3×flag+6×his) be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
  958. NP40 or Triton as membrane protein detergent - (reply: 1)
  959. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
  960. Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
  961. Introduction to x-ray crystallography of proteins - (reply: 1)
  962. Problem in transfer high MW protein - (reply: 5)
  963. precipitation of secreted protein from growth media - (reply: 1)
  964. No IgG Signals in immunostaining - urgent (reply: 2)
  965. how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
  966. dimerization or aggregation of my protein! - (reply: 3)
  967. GST-tagged protein binds to column but is not eluted - (reply: 1)
  968. IGg bands on SDS PAGE - (reply: 2)
  969. Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
  970. OKADAIC ACID for protein prep - (reply: 1)
  971. urea-denatured protein precipitates after dialysis - (reply: 1)
  972. Expressing 4 proteins in an operon - (reply: 6)
  973. Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
  974. Protein concentration for crystallization - (reply: 1)
  975. How can I decrease IgG background? - My IgG background is too high!!! (reply: 12)
  976. TCA and protein percipitation? - (reply: 2)
  977. Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
  978. Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
  979. Protein precipitates upon dialysis - (reply: 5)
  980. protein separation by filtration - (reply: 3)
  981. What is the epitope of acetylated tubulin antibody? - (reply: 6)
  982. Amount of chromatin for IgG control - URGENT! (reply: 1)
  983. membrane protein aggregation - (reply: 1)
  984. Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
  985. Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
  986. Protein Expression in stationary phase - (reply: 11)
  987. Protein Smearing - (reply: 2)
  988. after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
  989. His Tag Protein Purification - (reply: 6)
  990. Non-deacetylable Protein? - Is this possible? (reply: 3)
  991. Trouble about overexpression by lentiviral transduction - Why no protein of interest expressed!? (reply: 1)
  992. Amylose bound protein elution - (reply: 1)
  993. Protein elution from amylose resin - (reply: 1)
  994. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
  995. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
  996. Overexpressed protein not detected - Oh, the frustration... (reply: 1)
  997. Cellular proteins in medium - (reply: 1)
  998. Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
  999. Detecting phosphorylated Erk1/2 - (reply: 6)
  1000. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
  1001. yield vs concentration of protein - (reply: 3)
  1002. cys rich protein expression - (reply: 1)
  1003. insect protein expression - (reply: 1)
  1004. Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
  1005. protein extraction from tissue, help - (reply: 1)
  1006. Protein extraction from tissue - (reply: 1)
  1007. Transport & storage of protein extracts - (reply: 1)
  1008. Protein bands on the bottom of the gel - (reply: 5)
  1009. problem of membrane protein FPLC - (reply: 1)
  1010. my western blots of membrane protein do not work - (reply: 9)
  1011. IgM quantification - Monoclonal Antibodies (reply: 1)
  1012. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
  1013. Protein isolation from skin - (reply: 2)
  1014. How to concentrate my protein? - (reply: 1)
  1015. small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
  1016. Organism specific protein?? - data from maldi-ms (reply: 6)
  1017. buffer for membrane protein - (reply: 1)
  1018. Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
  1019. expression of His-tagged protein in insect cells - (reply: 2)
  1020. Whole cell lysate including nucelar proteins? - (reply: 3)
  1021. Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
  1022. Protein concentration and - (reply: 3)
  1023. membrane protein purification - (reply: 6)
  1024. VEGF-HEK - Protocol for collecting secreted protein (reply: 1)
  1025. Protein expression failing - (reply: 1)
  1026. How does high salt extraction of nuclear proteins work - (reply: 9)
  1027. Nuclear Protein Extraction - Nuclear Protein Extraction (reply: 1)
  1028. Dimerization of protein - (reply: 1)
  1029. Phosphorylated primers - (reply: 5)
  1030. Isolation of protein crosslinked to plasmid - (reply: 3)
  1031. Big differences between BCA and Bradford? - Two assays give very different results (reply: 8)
  1032. Contamination in GST fusion protein purification - (reply: 2)
  1033. Problem with protein samples - No bands detected at all on gel (reply: 2)
  1034. Protein localization - Protein localization (reply: 1)
  1035. moles —SS— groups in a protein calculation - (reply: 3)
  1036. Purification of scretory protein from bacteria - (reply: 2)
  1037. express protein with many rare codons - (reply: 1)
  1038. About GFP- tagged proteins in western blot - (reply: 1)
  1039. GST-tagged proteins - (reply: 1)
  1040. WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
  1041. lentiviral expression - transefection, low titier, protein expression (reply: 1)
  1042. No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
  1043. Protein Sample Preparation - Western Bloting (reply: 1)
  1044. defatting of soybean powder for protein isolation - defatting of soybean powder for protein isolation (reply: 3)
  1045. Protein precipitates during BrCN-sepharose coupling - (reply: 5)
  1046. Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 16)
  1047. Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
  1048. Protein Standard stability (BCA assay) - (reply: 3)
  1049. IHC staining of secreted proteins - (reply: 3)
  1050. Dimeric form of a protein? - I want my monomer! (reply: 2)
  1051. Ni-NTA his tag protein purification - (reply: 1)
  1052. Protein Soluble Test - Inclusion Bodies + UREA test? (reply: 3)
  1053. protein concentration factor - (reply: 3)
  1054. I want to buy p53 protein - which company? - (reply: 1)
  1055. Protein identification in 2D gel - (reply: 1)
  1056. Double Labelling of Same Protein - (reply: 4)
  1057. problem with lentiviral expression of protein - (reply: 2)
  1058. For those having problems with running & transfer of high MW proteins - (reply: 1)
  1059. Protein Size - (reply: 3)
  1060. why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
  1061. cloning of non-coding region for protein expression - protein expression (reply: 1)
  1062. Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
  1063. Transfer basic protein to PVDF - (reply: 1)
  1064. Protein expression - (reply: 4)
  1065. Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
  1066. single amino acid mutation effects on protein function - (reply: 1)
  1067. competition of thrombin and my protein on benzamidine beads - (reply: 5)
  1068. how to extract protein from agarose? - (reply: 1)
  1069. Protein expression with T7 promoter - (reply: 1)
  1070. disappearing high molecular protein and marker,thanks - (reply: 1)
  1071. I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
  1072. Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
  1073. co-IP of chromatin binding protein - (reply: 2)
  1074. protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
  1075. Protein cellular location tool - (reply: 1)
  1076. Western blotting for proteins in vesicles? - (reply: 2)
  1077. Protein expression using invitrogen pSecTag2 - (reply: 2)
  1078. Protein drag in IEF-WB - (reply: 3)
  1079. His protein expression problem - (reply: 1)
  1080. need help cloning cdna of a known protein - (reply: 1)
  1081. protein modelling - (reply: 1)
  1082. Help with Western Blotting of Keratin Proteins - (reply: 2)
  1083. Modification of Bradford Assay with SDS - THe attempt to increase sensitivity for a protein with SDS (reply: 1)
  1084. Mock IgG versus No Antibody - Negative Control - (reply: 7)
  1085. His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
  1086. protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
  1087. removal of SDS from protein - (reply: 4)
  1088. prepare sample protein for 2d from E.coli - (reply: 2)
  1089. Protein won't transfer for Western Blot - (reply: 5)
  1090. How topurify and measure stability of mAb IgM - (reply: 11)
  1091. PCR a plasmid protein - (reply: 2)
  1092. To find a protein's function which MW is 14kD. - (reply: 6)
  1093. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
  1094. protein oxidation - (reply: 1)
  1095. Protein size and gel percentage - (reply: 7)
  1096. how to explore protein interactions - (reply: 3)
  1097. Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
  1098. Protein precipitation by TCA acid + acetone - (reply: 4)
  1099. Non Reducing SDS protein ladder?? - (reply: 4)
  1100. biotinylation of proteins - (reply: 1)
  1101. Protein Free Blocking Buffers - (reply: 1)
  1102. More than one protein induced - (reply: 4)
  1103. Protein aggregates - Monomeric fraction varies with wavelength (reply: 4)
  1104. Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
  1105. protein concentration - can be measured at 280nm (reply: 3)
  1106. could FLAG-tagged protein be used in the Luc assay? - (reply: 1)
  1107. Exctinction Co-efficient - glycosylated proteins may be tricky (reply: 3)
  1108. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
  1109. Out of expected protein size in SDS-PAGE - (reply: 10)
  1110. Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
  1111. Mock (IgG) Ct Values - (reply: 3)
  1112. Storage of overexpressed protein - (reply: 4)
  1113. HELP_Bradford and protein problems - Different protein concentrations (reply: 1)
  1114. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
  1115. Protein present in Western, not ELISA - (reply: 10)
  1116. problem in the GST fusion protein purification - (reply: 4)
  1117. how to deal with protein precipitates during dialysis - (reply: 1)
  1118. reconstitution of membrane proteins into liposomes - (reply: 1)
  1119. Amount of inoculum? - for protein expression in E.coli (reply: 3)
  1120. ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)
  1121. the sensitive amount of protein for western blotting - (reply: 3)
  1122. Concentrating Protein - 8M Urea, Protein solution, concentrating (reply: 8)
  1123. Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
  1124. Qubit Protein Quantitation Issues - Qubit protein quantitation (reply: 2)
  1125. How to determine final amount of 20 ng protein per WB well - (reply: 8)
  1126. Protein extraction from plant leaves, bioactivity - (reply: 1)
  1127. High Molecular weight protein separation - (reply: 2)
  1128. Secreted protein for loading control - (reply: 2)
  1129. Protein Disappeareance - (reply: 1)
  1130. Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
  1131. Immunodepletion of high abundance protein - (reply: 1)
  1132. How to denaturate protein for ELISA - (reply: 1)
  1133. 2D gel - amount of protein (reply: 1)
  1134. protein size change after western blot - (reply: 1)
  1135. Coomassie blue before protein transfer - (reply: 9)
  1136. protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
  1137. epitope tagging of pcr products - (reply: 1)
  1138. densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
  1139. His tag Protein Elution - Elution Buffer (reply: 2)
  1140. net charge on protein - (reply: 1)
  1141. Can GST-tag be separated from GST-fusion protein - (reply: 1)
  1142. Protein analog - (reply: 1)
  1143. Increase in protein size - Increase in protein size (reply: 5)
  1144. How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
  1145. Membrane protein isolation - (reply: 1)
  1146. Expression of human protein in mice in-vivo - (reply: 3)
  1147. Help with T7RNAP purification - Cell-free protein expression (reply: 3)
  1148. step-wise dialysis? - (reply: 11)
  1149. membrane protein extraction buffer - (reply: 1)
  1150. Large protein transfer problem - (reply: 8)
  1151. Measuring protein aggregation using Non-denaturing PAGE - (reply: 3)
  1152. bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
  1153. Could I denature the proteins that were transferred on a membrane? - (reply: 3)
  1154. Quantiying bands. - Determination of protein contens (reply: 2)
  1155. Protein Sample Loading Buffer - (reply: 3)
  1156. Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
  1157. Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
  1158. shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
  1159. fusion protein construction - (reply: 1)
  1160. gfp-tagged protein not expressing- help! - (reply: 3)
  1161. pKa values of the protein - (reply: 1)
  1162. Plasmids for protein expression in mammalian cells - (reply: 2)
  1163. affinity purification and proteomics - (reply: 1)
  1164. Native PAGE w. Western protein marker - (reply: 4)
  1165. Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
  1166. Lyophilised protein - (reply: 3)
  1167. Usage of EST data for non-model species in proteomics - (reply: 3)
  1168. protein stuck - (reply: 3)
  1169. mRNA versus protein expression. Why inverse correlation? - (reply: 3)
  1170. A databse linking SNPs to functional consequences? - To the protein (reply: 2)
  1171. protein is degraded after purification - (reply: 2)
  1172. Protein qauntitation with nanodrop - SDS and urea (reply: 1)
  1173. Problem in protein expression - (reply: 2)
  1174. 24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
  1175. small protein blotting - (reply: 8)
  1176. What proteins stick to ProteinG on preclearing? - (reply: 3)
  1177. protein expression - Preparation of starter cultures (reply: 15)
  1178. help~ how concentrate protein solution in large-scale - (reply: 2)
  1179. Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
  1180. Problem with Protein elution using GST sepharose 4B - (reply: 1)
  1181. Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
  1182. how to isolate insoluble protein in bacterial induction? - (reply: 4)
  1183. Protein Concentration assay (Bradford assay) - (reply: 2)
  1184. protein digestion - (reply: 2)
  1185. Cleaving Fusion Proteins - (reply: 1)
  1186. In vivo monitoring leukemia growth - with green fluorescent protein or CFSE (reply: 3)
  1187. Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
  1188. Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
  1189. Re-dissolving of precipitated protein?? - (reply: 1)
  1190. Protein purification from Insect media - (reply: 1)
  1191. sticky proteins in co-IP - (reply: 1)
  1192. Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
  1193. protein/DNA Sequence Alignment - (reply: 1)
  1194. Purifying a strange native protein - (reply: 6)
  1195. Protein G Sepharose beads preparation - (reply: 2)
  1196. Ladder but no protein - (reply: 4)
  1197. Which test for cytokine production? - by PBMCs stimulated with proteins (reply: 6)
  1198. MIF protein secretion - Need help in knowing about MIF protein secretion (reply: 2)
  1199. Improving transient CHO protein expression - (reply: 2)
  1200. Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
  1201. Expression of a GFP-tagged protein - (reply: 2)
  1202. BCA assay for reducing ends - (reply: 2)
  1203. BCA assay for reducing ends - (reply: 1)
  1204. FPLC and Protein Chromatography - Resins, FPLC and protein chromatography (reply: 11)
  1205. Checking For Protein Expression - (reply: 3)
  1206. How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
  1207. Detecting Patterns of phosphorylated protein - (reply: 1)
  1208. Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
  1209. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  1210. insoluble protein fraction - (reply: 1)
  1211. Hyaluronic Acid Binding Protein 1 (HABP1) - I need some insight to prevent binding of this molecule to itself (reply: 2)
  1212. Newbie question: Stability of protein lystates - Freeze/thaw cycles affect phosphate groups? (reply: 2)
  1213. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
  1214. extracting protein from trizol - (reply: 2)
  1215. Blue Native Gel - denaturing proteins after transfer? - (reply: 9)
  1216. How much sonication power needed? - protein extraction from cells (reply: 2)
  1217. isolation of membrane associated proteins - (reply: 2)
  1218. Newbie question: how to determine protein concentration after IP? - (reply: 8)
  1219. protein extraction - from cell line in T25 flask (reply: 5)
  1220. Protein labeling calculation - (reply: 4)
  1221. Rabbit IgG purification - Rabbit IgG purification (reply: 5)
  1222. protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
  1223. Determining a protein's antigenic property - (reply: 4)
  1224. Oligo annealing - phosphorylation necessary? - (reply: 4)
  1225. how to design a protein interaction experiment - HELP (reply: 4)
  1226. Desalting protein - (reply: 7)
  1227. Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
  1228. How to check the translation? - His-tagged protein expression (reply: 8)
  1229. protein complex and siRNA - (reply: 2)
  1230. protein precipitation with TCA for dna extraction? - (reply: 1)
  1231. G Protein Coupled Receptors - (reply: 3)
  1232. Protein structure discovery - (reply: 1)
  1233. TCA precipitation_why protein pellet disapears after centrifugation? - (reply: 2)
  1234. Extraction of protein from sample buffer (+DTT) - (reply: 1)
  1235. Protein solubilisation for iTRAQ protocol/general protein solubilisation - (reply: 2)
  1236. protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
  1237. protein aggregation problem - (reply: 3)
  1238. protein precipitates out during concentrating it - (reply: 2)
  1239. precipitation during dialysis - (reply: 5)
  1240. Protein glycosylation - How to determine? (reply: 2)
  1241. Protein stability in RIPA buffer at room temp - (reply: 3)
  1242. Protein synthesis inhibition - (reply: 4)
  1243. His tag protein purification - (reply: 3)
  1244. How to extract protein from SDS-PAGE gel? - (reply: 7)
  1245. how to put in evidence protein after IP - (reply: 2)
  1246. GST-fusion protein purification - How to purify it? (reply: 9)
  1247. protein extraction from primary cells - (reply: 1)
  1248. Extracting proteins from cells - (reply: 1)
  1249. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
  1250. amount of proteins in urine - (reply: 12)
  1251. Help_Role of BSA for the protein reconstitution - (reply: 5)
  1252. BCA Protein Assay Problems - (reply: 1)
  1253. Protein lysis buffer - (reply: 4)
  1254. IP low protein and MS - (reply: 1)
  1255. protein degardation during gel filteration - (reply: 2)
  1256. Protein solubility improvements? - any methods? (reply: 5)
  1257. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
  1258. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
  1259. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
  1260. Immunolotting of actin protein coming at higher MW - (reply: 1)
  1261. Isolation of secreted proteins from culture media - (reply: 2)
  1262. proteins stuck at interface between stack and separating gel - (reply: 2)
  1263. Protein loading marker for glycoproteins - (reply: 2)
  1264. Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
  1265. distinguish protein expression from high homology - (reply: 1)
  1266. Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
  1267. Lost my proteins ! - (reply: 3)
  1268. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
  1269. Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
  1270. Alternative methods to detect protein:protein interaction - (reply: 3)
  1271. protein overexpression question. - (reply: 4)
  1272. Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
  1273. Protein targetting - (reply: 1)
  1274. FCS and Protein assay - (reply: 3)
  1275. Simultaneous BLAST for several proteins - (reply: 4)
  1276. Problems in His-tag protein purification with TALON - (reply: 1)
  1277. protein quantification by bradford - (reply: 3)
  1278. protein extraction from tumor samples containing matrigel? - (reply: 2)
  1279. Extracting Proteins From Soil - (reply: 3)
  1280. Endogenous protein purification - (reply: 2)
  1281. strategy to isolate cDNA and express protein - (reply: 1)
  1282. Amount of protein loaded per well? - (reply: 4)
  1283. signal peptide search - How to find subcellular localization signals in a protein sequence (reply: 1)
  1284. Protein in and out of solution - (reply: 1)
  1285. Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
  1286. taking protein out of membrane - (reply: 1)
  1287. His Tag protein - (reply: 4)
  1288. Contaminating proteins for Fluorescence polarization assay - (reply: 1)
  1289. how to dialysis against acetic acid - (reply: 1)
  1290. protein extraction after cell lysing - (reply: 2)
  1291. Protein extraction from rat aorta - (reply: 3)
  1292. Normal IgG background - (reply: 2)
  1293. Making Recombinant Proteins - (reply: 2)
  1294. sensitive protein quantification assay - BCA not helping (reply: 3)
  1295. sensitive protein quantification assay - (reply: 2)
  1296. GST- protein, batch purification - no protein after elution (reply: 3)
  1297. WB does not correspond to protein array - (reply: 1)
  1298. Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
  1299. Co-IP problem - Weird IgG band (reply: 1)
  1300. No protein expression - (reply: 3)
  1301. Purifying hydrogen bonded peripheral protein - (reply: 3)
  1302. How to elute this protein?! - (reply: 5)
  1303. Recombinant protein or peptide? - (reply: 1)
  1304. GST-tag protein purification - GST-tag protein purification (reply: 4)
  1305. strength of protein interaction for co Ip - (reply: 1)
  1306. Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
  1307. Stability of Biotin labelled protein?? - (reply: 1)
  1308. Isolation DNA and protein from a single sample - (reply: 5)
  1309. How to purify a transmembrane protein from others proteins? - (reply: 1)
  1310. Protein structure - (reply: 1)
  1311. Dialysis against PBS of IgG/IgA - a bit confusing (reply: 3)
  1312. simultaneous extraction of nucleic acids and proteins - (reply: 3)
  1313. Protein tags - (reply: 2)
  1314. Could denatured proteins be stored at -20°C (or -80°C) until use (for SDS-PAGE)? - (reply: 3)
  1315. truncation of protein expression - (reply: 1)
  1316. SDS Page Help - using purified protein (reply: 3)
  1317. PROTEIN PRECIPITATION SOLUTION - (reply: 1)
  1318. protein precipitation - (reply: 3)
  1319. GST fusion protein purification - (reply: 1)
  1320. Integral protein question - (reply: 2)
  1321. storage of protein samples - (reply: 1)
  1322. unstable protein expression - (reply: 1)
  1323. DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
  1324. splice variant encoding same protein - (reply: 1)
  1325. B-per GST fusion protein purification kit - (reply: 2)
  1326. Endogenously Biotinylated Proteins - (reply: 3)
  1327. protein binding site prediction - (reply: 2)
  1328. gradient to separate 1kDa proteins - (reply: 3)
  1329. Protein precipitates out so fast - (reply: 5)
  1330. Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
  1331. Principles behind DNA RNA protein isolation and purification - (reply: 2)
  1332. Lysis Buffer for Total Protein Assay - (reply: 1)
  1333. Number of genes and number of proteins in humans - (reply: 1)
  1334. automated protein structure search - (reply: 4)
  1335. "Gentle" IgG elution - (reply: 1)
  1336. Insert phosphorylation upon Klenow? - (reply: 5)
  1337. Protein Conc determination - (reply: 2)
  1338. protein stability - (reply: 3)
  1339. pQE vector protein expression - (reply: 3)
  1340. Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
  1341. Reconstitution of protein standard - (reply: 3)
  1342. Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
  1343. protein size gradient gel - (reply: 10)
  1344. A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
  1345. lyophilized protein now looks clear - (reply: 3)
  1346. Playing for keeps - Reusing remaining protein sample (reply: 3)
  1347. isoelectric point of protein - (reply: 1)
  1348. GST-Tagged protein - (reply: 4)
  1349. Effect of sonication on membrane anchored proteins - (reply: 1)
  1350. Methods to determine the oligomeric state of a protein - (reply: 1)
  1351. Problem concerning protein quantitation - (reply: 2)
  1352. Protein concentration - (reply: 4)
  1353. Getting rid of background protein binding on GST beads - (reply: 5)
  1354. denaturing proteins in gel - (reply: 1)
  1355. Protein modification search - (reply: 1)
  1356. Protein degradation - (reply: 2)
  1357. problems with detecting protein expression - protein expression in cell (reply: 4)
  1358. yeast two hybrid - are protein interactions species specific? (reply: 1)
  1359. Problem with protein precipitation - (reply: 1)
  1360. Software to create protein maps - (reply: 1)
  1361. Urea and protein storage - (reply: 6)
  1362. Who know anything about dialysis on protein extractions ? - (reply: 7)
  1363. Problem assessing protein concentration - Protein concentration (reply: 4)
  1364. Protein doesn't bind any column except Ni - (reply: 10)
  1365. Phosphoproteins staining - (reply: 2)
  1366. IgG control for immunopercipitation - (reply: 1)
  1367. quantification of Biotinylated protein ?? - (reply: 3)
  1368. Problems with large protein - (reply: 3)
  1369. protein concentration method - want really high yield (reply: 4)
  1370. protein structure prediction - (reply: 1)
  1371. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
  1372. stabilization of purified protein solutions - (reply: 2)
  1373. Rcovery of proteins from gels - (reply: 2)
  1374. Protein much larger on Western blot than predicted - (reply: 12)
  1375. Showed a bigger and broad smear of protein!! - (reply: 4)
  1376. Proteins not running till the end of the gel - (reply: 2)
  1377. The maximum resolution obtained by crystallography? - Can we determine the primary structure of a protein by that? (reply: 3)
  1378. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
  1379. mutation in secreted protein - (reply: 5)
  1380. Recombinant Protein migration - Doubts on protein migration (reply: 5)
  1381. How does the protein fixation on SDS gel work? - (reply: 3)
  1382. Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
  1383. SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
  1384. ChIP-CHIP IP vs IgG vs Unbound vs Input - (reply: 2)
  1385. Mammalian protein expression in E. coli - (reply: 1)
  1386. Immunoprecipitation - Multiple proteins from single lysate (reply: 2)
  1387. can beta-catenin be used as a house keeping protein? - (reply: 3)
  1388. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
  1389. Protein extraction from cell culture media - (reply: 9)
  1390. protein precipitation - (reply: 1)
  1391. brown pigment attached to the protein - (reply: 3)
  1392. Protein extraction from mouse organs - (reply: 6)
  1393. no expression of my protein - (reply: 1)
  1394. confirming nucleotide state of isolated G protein - (reply: 1)
  1395. Tagging a protein with a nucelar export signal - (reply: 2)
  1396. part of protein sequence missing - (reply: 4)
  1397. protein disapeared from gel! - (reply: 11)
  1398. Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 7)
  1399. Phosphoproteins - (reply: 1)
  1400. Mouse Urinary Protein Quantification - BCA possible (reply: 2)
  1401. How to define the domans in protein sequence - (reply: 3)
  1402. protein purification - (reply: 5)
  1403. protein concentration - how to determine the protein concentration very accurately?? (reply: 2)
  1404. IgM hybridoma protocol - Protocol targeted for isolation of IgM secreting hybridomas (reply: 5)
  1405. Slimy protein lysates - (reply: 3)
  1406. protein band size determination - any software available? (reply: 2)