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Protein Related Discussions
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How to get pure protein of a gene - (reply: 1)
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Homogenisation of protein - (reply: 1)
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How to get soluble protein? - (reply: 3)
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protein concentration/well calculation - (reply: 1)
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How to get pure protein of a gene - (reply: 3)
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IPTG induced protein go to inclusion bodies - (reply: 1)
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'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
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Protein Isolation from mouse brain: problems - (reply: 2)
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Protein extraction from dead cells - (reply: 1)
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Problems with high molecular weight protein transfer in western blot - (reply: 1)
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Expression level analysis at mRNA vs protein level - (reply: 6)
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Trouble Western Blotting a 360kDa Protein - (reply: 4)
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Purifying recombinant protein from pellet? - (reply: 2)
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Protein ladder - (reply: 1)
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Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
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Question about the amount of mRNA and secreted protein level!!! THX~ - (reply: 1)
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Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
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Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
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membrane protein expression - (reply: 1)
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normalization to protein content or cell number - (reply: 2)
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protein solubilization and quantitation after extraction using Trizol - (reply: 2)
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Blunt-ligating dephosphorylated insert into phosphorylated vector - (reply: 5)
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Magnesium acetate in protein lysates - (reply: 1)
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detection of phosphorylation from cell pellet - (reply: 2)
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How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? - (reply: 6)
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Protein sequencing gaps - (reply: 1)
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which protein can I use as a loading control for nuclear protein fraction? - (reply: 2)
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Suggestions for experimental or in silico validation related to ncRNA or protein - (reply: 1)
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desired protein is going in pellet...do not want to go for renaturation - (reply: 3)
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Concentrating protein of a particular size - (reply: 1)
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Extra bands after protein expression/purification - (reply: 4)
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96 well protein expression - (reply: 1)
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Different cell lines different protein stability - (reply: 1)
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Better protein Expression? - (reply: 2)
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Anti-human IgG phosphatase give negative results - (reply: 13)
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protein extraction from transwell inserts - (reply: 2)
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Expression of protein with pI 8.2 - (reply: 1)
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Failed BCA protein assay, white crystalline solid after speedvac - (reply: 1)
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Methionine for protein expression - (reply: 3)
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Protein expression in yeast - Yeast Genotype - (reply: 1)
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Carrier protein concentration - (reply: 2)
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protein - (reply: 1)
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Anti-his antibodies - Noob to protein work - (reply: 10)
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Protein localization studies - (reply: 5)
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Protein Expression problems - (reply: 4)
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Cell Envelope Proteins Smeared - (reply: 1)
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Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection - (reply: 2)
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Problem with merged bands within western blot caused by amount of protein loaded - (reply: 7)
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predict possible protein protein interaction - (reply: 1)
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Cellular localisation of a protein - (reply: 1)
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Sequential phosphorylation in vivo but not in vitro - (reply: 4)
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problem in expressing A DNA binding protein - (reply: 1)
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Detection of glycosylations on Western blotted proteins - (reply: 2)
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Problems with protein estimation and western blots - (reply: 1)
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histone proteins extraction and 2D - (reply: 1)
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Quantity of protein to charge - (reply: 1)
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Determining the N- and C-terminal regions in a protein - (reply: 6)
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Properties of amino acids and protein functional activity - (reply: 1)
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Phosphorylation - (reply: 2)
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Protein tagging with GFP/effect of expression construct - (reply: 2)
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RNAi problem - Gene is knocked down, but not protein - (reply: 5)
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Insoluble Protein purification - (reply: 1)
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Does DNA have to be supercoiled for transient protein expression? - (reply: 4)
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my protein bands and protein marker becomes abnormally clearer sometimes - (reply: 3)
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BLASTing protein block - (reply: 1)
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IgG control - (reply: 6)
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Difficulty in expressing fusion protein - (reply: 1)
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IgG control - (reply: 4)
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Native PAGE, interaction of proteins, validity - (reply: 4)
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what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
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Peptide Protein Conjugation - (reply: 2)
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Same non specific band for different target proteins - (reply: 1)
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Types of 96-well plates used for BCA protein quantification - (reply: 1)
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Protein measurements in 2% Triton X samples - (reply: 1)
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No protein in cytoplasmic extract - (reply: 1)
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Size and composition of spacer between protein and tag? - (reply: 4)
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Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
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protein extraction from pirmary keratinocytes - (reply: 2)
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his tagged protein - (reply: 1)
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How to purify unstable protein? - (reply: 10)
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unstable flag tagged protein - (reply: 4)
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Reusing scrapers + needles & syringe for total protein isolation - (reply: 1)
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SDS-PAGE of negatively charged protein - (reply: 4)
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GFP tagged protein expression detection - (reply: 1)
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How to obtain high-level production of low molecular weight recombinant proteins - (reply: 1)
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How to quantify the protein amount for serum samples ? - (reply: 1)
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Insoluble Protein - (reply: 5)
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making recombinant protein - (reply: 2)
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Problem with expression of recombinant protein - (reply: 3)
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how to make a recombinant protein with signal peptide - (reply: 1)
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Large protein blot ~400kDa - (reply: 3)
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Protein folding affected by neg. cahrges amino acids? - (reply: 4)
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getting rid of imidazole in purified protein? - (reply: 2)
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which gel concentration can separate the 7.5 kDa protein? - (reply: 6)
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Western Blotting of Proteins less than 10 kDa - (reply: 1)
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Protein wight in kDa - (reply: 2)
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BN-PAGE of purified membrane associated protein - (reply: 5)
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Expression of protein X varies in different w.b running (in one cell line, but - (reply: 3)
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Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
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Mitochondrial and cytosolic protein isolation and purification - (reply: 1)
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Problems eluting a GST fusion protein in GSTrap FF - (reply: 6)
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My protein samples are running abnormally on a SDS PAGE - (reply: 3)
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Fusing permanently a phosphate molecule to a protein. - (reply: 4)
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Bacterial expression: incorporating radioactivity into overexpressed protein - (reply: 1)
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Can proteins with sizes smaller than 60 kda be seen as bands in a 6% gel western - (reply: 3)
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Protein of 160 kda is present on 6% gel but gapdh housekeeping protein is not. - (reply: 6)
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ChIP-PCR: Amplification in IgG negative control - (reply: 1)
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Precipitations in protein dialysis - (reply: 1)
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mRNA increase and protein expression - (reply: 10)
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large protein - (reply: 3)
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applying cell culture protocol of protein extraction for rat intact tissue is co - (reply: 1)
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A problem with IgG purification by Protein G chromatography - (reply: 4)
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problem with loading the protein on to SDS-Gel - (reply: 1)
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anti-GLUT4 antibody, extracellular epitope? - (reply: 1)
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Protein contamination with DNA - (reply: 5)
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Western blot not showing protein - (reply: 2)
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Western Blot not showing protein - (reply: 4)
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No activity of fungi protein in E.coli - (reply: 15)
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longer running of proteins in SDS PAGE! - (reply: 2)
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Rehydrating/Resuspending Drosophila Proteins - (reply: 1)
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How exactly does an IgG antibody control in ChIP work? - (reply: 3)
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PacI affect protein translation? - (reply: 1)
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ELISA Kits for proteins in brain homogenates - (reply: 6)
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Do we really need to do protein quantification - (reply: 4)
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Maximum number of proteins - (reply: 1)
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protein did not knock down by shRNA, but RNA did knock down - (reply: 4)
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Western Blot for 320KD Protein - (reply: 4)
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Can someone recommend a good proteomics company/facility with Orbitrap and can p - (reply: 1)
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Dot Blot - Protein not adhering to membrane - (reply: 1)
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Smears after protein precipitation - (reply: 4)
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protein expression level and OD600 in E.coli - (reply: 1)
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Protein contamination - How to remove all protein from my DNA? - (reply: 3)
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FACS Sorting and Protein Purification - (reply: 5)
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How to extract total protein from gram negative bacteria for SDS page and BCA an - (reply: 2)
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biotinylated protein extracts and antigen-antibody stringency - (reply: 3)
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overexpression FLAG-tagged protein shows extra bands in WB - (reply: 2)
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Protein purification buffer - (reply: 3)
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Protein purification in native conditions - (reply: 1)
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Membrane proteins - (reply: 6)
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Protein extraction efficiency - (reply: 2)
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Triton X-100 and Dialysis - (reply: 2)
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homology proteins - (reply: 1)
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Improving expression of recomb proteins in baculovirus - (reply: 1)
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Inhibition of Protein Synthesis in Eukaryotes - (reply: 1)
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Trizol and Protein extraction - (reply: 2)
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Protein Extraction SDS-Buffer Pellet not Dissolving and Laemlli Bufffer Green? - (reply: 3)
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Do amino acids and his tag on vector affect protein of interest? - (reply: 4)
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Western Blot Two Proteins - (reply: 1)
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Western Blot Protein Length - (reply: 4)
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Increase toxic protein expression? - (reply: 1)
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Recombinant protein from cell lysate - (reply: 4)
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Gooey DNA is protein samples after adding sample buffer - (reply: 1)
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pET47b proteins not expressing in BL21 (DE3) - (reply: 5)
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Thrombin removel His tag protein digestion - (reply: 1)
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protein expression vector.. - (reply: 4)
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ChIP-Seq with HA-tagged protein - (reply: 1)
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Fc fusion protein smearing on SDS PAGE - (reply: 2)
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protein solution cloudy after dialysis - (reply: 5)
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Using protein content to standardize ELISA data? - (reply: 2)
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How to improve protein solubility in E. coli? - (reply: 10)
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RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
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methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
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type of IgG in my sample?? - proteinA purified sample (reply: 1)
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To isolate DNA, RNA and Protein from same culture flask for methylation study? - (reply: 2)
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Help needed with stable cell line creation - problems making a cell line expressing a viral protein (reply: 3)
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Protein structure prediction - (reply: 2)
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Help with small protein with his-tag purification - (reply: 7)
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protein precipitate at pI?? - (reply: 1)
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Protein agregation in WB samples - (reply: 3)
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Lentiviral transduction, no target protein expression and lost other reporter - (reply: 1)
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Protein dissolved - After acetone precipitation (reply: 2)
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Max amount of protein - (reply: 4)
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TCA/Acetone protein precipitation - (reply: 9)
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how to add 3XHA to the N-terminal of a protein - (reply: 5)
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Separating protein isoforms and quantifying activity - (reply: 3)
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Protein refolding - (reply: 2)
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Freezing proteins at -80 - (reply: 1)
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Overrepresentation of genomic region in ChIP IgG control?! - (reply: 2)
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Discrimination against denatured proteins?! - Can ELISA discriminate agains denatured proteins? (reply: 2)
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Chromatin Protein IP - (reply: 2)
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How can I detect mannosidase activity of a protein - (reply: 1)
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Protein Aggregation with 6xHis tagged CreGFP - (reply: 1)
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Problem with protein resolving in electrophoresis - (reply: 7)
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Protein stability at room temperature - (reply: 3)
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Help, problems with expressing human proteins in E.coli - IPTG induced cells turn pink (reply: 1)
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protein pass through column - (reply: 1)
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Help with protein expression - how to optimize expression of large GST fusion protein (reply: 4)
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protein precipitation - isolation of proteins (reply: 1)
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effect of lysozyme on gst-fusion protein - (reply: 1)
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Protein purification from HEK 293 cells - (reply: 1)
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Odd result help - Luciferase / protein / mRNA levels (reply: 1)
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Upscaling protein expression in E.coli - (reply: 2)
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Dot-blot with immobilized ABs on membrane for protein detection - (reply: 8)
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confirm proteins interacting with histones using acid extraction - (reply: 4)
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Housekeeping protein issues - Is my experiment affecting my housekeeping protein levels? (reply: 1)
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nuclease contamination in protein prep - (reply: 4)
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Bio Rad protein assay for liver homogenate - (reply: 1)
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protein purification - (reply: 1)
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Bradford Vs beta-actin for WB protein quantification - (reply: 3)
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Dual luciferase assay - proper controls? - LUC below pGL3 despite protein activity (reply: 4)
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SDS Page heat up and "fry" the gel and protein (Western Blot) - (reply: 2)
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acrylamide gel for low molecular weight protein - (reply: 2)
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ELISA kits protocols, antibodies, control proteins, and more - (reply: 1)
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HELP! TISSUE STORAGE AND PROTEIN DEGRADATION! - (reply: 1)
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Pierce BCA Assay problem - Protein values bizarre (reply: 3)
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plant protein purification opinion - (reply: 3)
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WB - Transferring REALLY big proteins - (reply: 2)
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Unable to Purify protein! - Protein purification (reply: 2)
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Cloning and expressing a mystery 71KDa protein - (reply: 9)
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HIS-tagged protein purification - (reply: 12)
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how to elute "only" my recombinant protein - final elutes comes with non-specific proteins... (reply: 4)
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Is it possible to islate strong negative charged protein - (reply: 1)
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Protein expression vectors Please help - (reply: 2)
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what is protein flexibility - (reply: 1)
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His-tagged protein purification problem - (reply: 2)
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protein level expression - (reply: 1)
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How to get rid of DNA/RNA in protein expression preps - Is there an alternative to DNAse? (reply: 1)
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Looking for protocol to do biotinlynation on each single protein in cell lysate - (reply: 1)
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proteins, new ones? - How to know (reply: 8)
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KO mice show band for knocked-out protein - (reply: 1)
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Trouble in protein quantification - (reply: 2)
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SDS-PAGE gel wont separate small proteins - (reply: 5)
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LIC cloning - pETDUet LIC insertion for dual protein expression (reply: 2)
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Unexpected expression of my protein in HepG2 - (reply: 3)
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Protein Aggregation, Sample Boiling, help! - Western Blotting (reply: 4)
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EXPRESSION PROBLEM IN GST TAGGED PROTEIN - (reply: 1)
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Gene Synthesis - Navigating through vectors, copy number, toxic protein (reply: 4)
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GST-fusion protein degrades in-vivo - (reply: 3)
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Purification of recombinant proteins from insect cells - (reply: 2)
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Non reducing protein ladder? - (reply: 5)
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similar size of proteins but different movement on SDS-PAGE - (reply: 1)
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Protein Expression in yeast - protein not expressed (reply: 1)
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SDS-PAGE: Effect of Acrylamide concentration - Is it different for high and low MW proteins (reply: 2)
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no detection of proteins at 25KDa - (reply: 4)
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help with protein elution - (reply: 1)
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Protein quantification after 8M urea lysis - (reply: 3)
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phosphorylted protein smaller not bigger? - (reply: 1)
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protein phosphorylation status in sample buffer - (reply: 2)
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Protein detection - western blot (reply: 3)
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Boiling, protein aggregation......reversal?? (western) - (reply: 3)
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expression of protein - (reply: 1)
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Tag proteins for CO-IP and confocal - (reply: 1)
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insoluble protein expression help - (reply: 3)
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Active protein extract from corn - (reply: 2)
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Model Protein - Need Advice! - (reply: 3)
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You have truncated protein domain that won't express? - If you wanted to express a protein but failed because domain not intac (reply: 1)
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CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
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His-tag protein vs. BSA - (reply: 3)
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Thrombin cleaved GST-tagged protein remained bound to beads! - GST protein purification Thrombin (reply: 7)
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YFP protein fusion accumulates - (reply: 4)
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Protein Concentation from M to mg - (reply: 2)
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Storage condition of Recombinant protein (native) - suggestions? (reply: 5)
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Problems in recombinant protein expression - Problems in recombinant protein expression (reply: 5)
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protein interactions - (reply: 2)
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MTT assay, protein assay analysis - Need assistance with analysis of these two assays (reply: 6)
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Lactobacillus crude protein extraction? - (reply: 8)
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Problem of recombinant bacterial membrane anchored protein - Recombinant protein (reply: 2)
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Western Blot of Large Protein Help - (reply: 1)
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Fusion tagged protein and Western Blot problem - (reply: 3)
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Compatible detergents for proteomics - (reply: 4)
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Troubleshooting Protein Purification using FPLC - (reply: 1)
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how to make protein soluble? - protein solubility (reply: 4)
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Quantification of proteins in Western Bands - (reply: 2)
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protein yield is very low after purification - protein purification (reply: 9)
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his- & biotin- tag protein purfication - (reply: 2)
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How to remove protein to show an activity is protein based - (reply: 7)
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FZR1 PROTEIN EXPRESSION - (reply: 1)
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help with his tagged protein purification - precipitation problem (reply: 1)
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Overexpression plasmid is not expressing protein - (reply: 11)
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Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) - (reply: 1)
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why phosphorylated protein relative to total protein? - (reply: 2)
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SDS-PAGE with 500 ug protein - (reply: 3)
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Heterologous protein extraction in yeast for enzymatic activity test - (reply: 5)
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His tag protein purification - (reply: 7)
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Dry protein gels without cracking - (reply: 5)
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Backgrounds persistence and higher than target protein in western blot - Expressed in BL21(DE3) (reply: 14)
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insoluble fusion protein - (reply: 1)
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Protein precipitating need help choosing suitable buffering system - (reply: 3)
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Total Cell protein - (reply: 1)
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ammonium precipitation of Proteins - (reply: 2)
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Expressing human protein in E.Coli - (reply: 12)
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Assay in Blood - sticky protein (reply: 13)
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dye protein ladder - (reply: 5)
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protocol to transfer protein on transfer membrane fluoride bind - please tell me how to transfer proteins from sds page (reply: 4)
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Gel gets squiggly bands for low molecular weight proteins - (This is driving me insane) (reply: 3)
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Naming system of IgG and Identification of IgG subclass - (reply: 5)
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How do you identify protein detected on western blot from a lysate???? - (reply: 2)
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how to express protein in soluble form - (reply: 1)
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Help with GST protein purification - (reply: 4)
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Antiobiotics for protein expression - (reply: 3)
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Number of proteins per cell? - One of THESE questions... ;) (reply: 1)
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IP no target protein in elution - (reply: 1)
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protein function prediction - (reply: 5)
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stabile transfection, all have high mRNA level, only ~ 50 % have protein - (reply: 1)
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Protein stability - (reply: 4)
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PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
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Protein isolation in zebrafish - (reply: 1)
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how to extract proteins from HEK293 - I need your experience (reply: 3)
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Identification of purity of the protein - (reply: 2)
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ChIP - protein contamination of ChIP'ed material - (reply: 4)
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Biotinylation of protein in tris buffer - (reply: 2)
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Maltose Binding Protein Sequence - A sequence for the Maltose Binding Protein. (reply: 1)
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HELP!?!? with MEMBRANE PROTEIN extraction - Extraction of GPCRs from HEK293T for IB and IP (reply: 1)
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Protein kinase protocols - (reply: 2)
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Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
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Purification of His tagged protein - (reply: 2)
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BCA diluent and standard curve - (reply: 1)
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protein induction problem - (reply: 4)
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Loss of protein expression following double transformation - Protein expression post transformation (reply: 10)
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Protein purification. How to make function of recombinant protein? - (reply: 2)
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Is My Protein Pure or Not? - Protein purification, SDS-Page (reply: 19)
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Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
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ChIP doesn't work. Please help! - Too much "normal rabbit IgG" bockround (reply: 8)
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Protein expression declines after overnight incubation - (reply: 4)
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Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
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elimination of Protein interference from DNA - for RT-PCR (reply: 2)
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Fusion protein linker - (reply: 2)
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common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
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Homogenization of articular cartilage prior to protein extraction - (reply: 1)
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Protein Phosphorylation - (reply: 2)
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Western for very small protein - (reply: 3)
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Protein streaking on Blue Native-PAGE - (reply: 9)
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basic questions about protein assays and cuvettes - (reply: 4)
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His-Tag protein purification:protein eluted with NaOH??? - (reply: 3)
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antibody production-how to differentiate epitope as foreign - (reply: 4)
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How to attain pure chains of one protein complex - (reply: 1)
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MINI Dialysis Units - below 2K MWCO? (reply: 1)
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Help for co-transfection of proteins and plasmid - (reply: 1)
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Problems with precipitation of proteins using TCA/aceton - (reply: 3)
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Fusion Protein - (reply: 3)
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Solubility of proteins - (reply: 1)
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Loss of protein upon concentration - (reply: 4)
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protein overexpression in HeLa cells - (reply: 1)
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His-tag protein purification - aggregation of protein (reply: 5)
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After inducing with IPTG for protein expression, my cell pellet after centrifuga - (reply: 1)
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CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
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High mRNA but no protein expression - (reply: 10)
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Protein Purification of an His-Tag Protein using a Ni Column - (reply: 1)
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Baculovirus Expert Needed (Occlusion bodies) - Can proteins become stuck in occlusion bodies in baculovirus? (reply: 6)
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Decrease in concentration after protein purification - (reply: 3)
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Circular Dichroism and disordered proteins - (reply: 1)
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2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
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Bacterial Induction: Same concentration of protein samples? - (reply: 1)
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protein phosphorylation - methods for protein phosphorylation (reply: 9)
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Unable to get proper PCR amplification - PCR does not correlate protein expression (reply: 3)
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labeling a protein - (reply: 3)
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Co-IP of secreted proteins - (reply: 1)
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protein protein interaction discovery - (reply: 1)
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what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
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Problem in stable expression of target protein using lentiviral vector - (reply: 3)
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How to be good at prep? - Calling all protein experts (reply: 2)
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Protein Migration Problem [S.O.S.] - Moves in SDS-PAGE but not in Native (reply: 8)
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His-tagged protein purification - (reply: 3)
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143 kDa protein - (reply: 3)
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Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
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protein purification - (reply: 3)
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Purification od insoluble protein (TIMP3) - (reply: 2)
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eluting gst fusion proteins - (reply: 1)
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How to pull down chromatin and identify protein associated with it? - (reply: 1)
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contamination in protein expression - I got a couple of questions (reply: 3)
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Protein extraction from perfusion fixed brain tissue - (reply: 5)
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Always expressing truncated protein - (reply: 3)
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Antibody production. - Discrimination between almost identical proteins. (reply: 1)
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secreted protein quantification - (reply: 2)
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protein sample degradation - (reply: 7)
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Major Protein Loss! Why? - (reply: 2)
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Protein in the nucleus vs in the cytoplasm - (reply: 1)
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Over Experssion of A Protein - (reply: 3)
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Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
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problem in protein purification - (reply: 5)
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Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
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Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
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protein purification - (reply: 5)
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Cloning the coding region of a protein - (reply: 4)
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Problem with protein purification By FPLC - (reply: 4)
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Molar ratios of IgM and IgG antibodies in in vitro assays - (reply: 3)
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cytoplasmic and nuclear extraction for BCA - (reply: 6)
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Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
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EGFP protein location problem fixing with PFA - (reply: 3)
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Isolating bacterial RNA and protein from the host tissue sample - (reply: 3)
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protein prep - about prptein preperation in diagnosis (reply: 1)
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Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
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loss of protein during concentration - (reply: 6)
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protein degradation - (reply: 3)
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Cell lysates - Protein precipitation? - (reply: 3)
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Protein precipitation with TCA? - Protocols, suggestions, ideas, experiences (reply: 2)
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His-tag proteins not eluted from Ni-Beads - (reply: 2)
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Is CNBr activated Sepharose good for binding IgG? - (reply: 3)
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Western Blots on Membrane Proteins - (reply: 9)
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Samples for Western to detect phosphorylation of EGFR - (reply: 2)
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quantification of a protein in tissue - (reply: 3)
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protein sequencing company - (reply: 2)
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Protein extraction from mouse prostate - (reply: 3)
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Help - proteins faster than the dye front - (reply: 8)
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fluorescent proteins expressed in nucleus - (reply: 6)
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IGg or F(ab´)2 Fragment of IgG - HRP-conjugated secondary antibody (reply: 1)
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A suitable vector for the cloning of multiple genes for protein expression - (reply: 4)
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Using FASTA Seq to Compare protein Seq. - (reply: 1)
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Protein SDS-PAGE thick bands / smear - (reply: 4)
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Denaturing protein before blotting - (reply: 1)
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not completed transfer proteins on NT membrane - western blot (reply: 2)
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trouble with protein expression of high AT rich gene - (reply: 1)
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No protein lane have bands on Coomassie and western blot?! - (reply: 4)
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Protein cleavage and neurodegeneration. - (reply: 1)
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Extraction protein from paraffin-embedded tissues - (reply: 1)
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column chromatography - elution buffer for proteins (reply: 2)
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Lost protein after gel filtration - Gel Filtration (reply: 6)
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protein size - (reply: 1)
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is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
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New smeared bands for protein that previously ran fine - Smeared protein bands now but not the previous 50 times I've run same (reply: 1)
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Weird effect of siRNA on protein in cells - (reply: 3)
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estimate size of protein - (reply: 2)
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Protein elution from GST-fusion protein complex - (reply: 3)
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Problems with Soluble and Insoluble Protein Fraction - (reply: 4)
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total cell protein vs membrane protein - (reply: 4)
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amount of plasmid for transformation and fusion protein expression - (reply: 1)
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gst fusion protein - (reply: 3)
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Biological process from protein list - (reply: 3)
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how to get pure protein - (reply: 1)
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Liposome + IgG sample in SDS-PAGE - (reply: 1)
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Different centrifugation duration, different protein?? - (reply: 1)
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Protein tags - (reply: 6)
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What portion of protein can I get under this protocol? - (reply: 3)
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SDS-PAGE for proteins which have pI>7 - (reply: 1)
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looking for kinase inhibitor to study phosphatase - kinase inhibitor (Inhibit phosphorylation on transmembrane receptor) (reply: 4)
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to get protein in cytosol from inclusion bodies - (reply: 4)
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How can I get protein in soluble fration - (reply: 2)
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Looking for precipitation method to reduce the volume of my protein complex elu - TCA precipitation seems to be hard to be resolved again (reply: 6)
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How long does it takes to make a crystallography of a protein? - (reply: 1)
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how to detect a knowen protein's receptor? - (reply: 1)
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Is IgG monoclonal antibody better then IgM? why? - why everyone prefer IgG? (reply: 3)
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protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
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How much protein from a confluent 6 well plate? - (reply: 5)
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Protein expression - (reply: 8)
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Protein quantification - (reply: 2)
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immunochemistry - using cofocal to identify two protein localization (reply: 1)
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Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
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Wet Transfer is not transferring 300kDa protein, why? - problems with wet transfer (reply: 11)
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Bands are lower than actual protein - (reply: 1)
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Protein lysate storage - (reply: 2)
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to bring protein in soluble fraction - my protein is cysteine rich and i am getting it in insoluble fraction. (reply: 1)
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Tricine SDS-PAGE for plant mitochondria - Membrane proteomics (reply: 1)
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Protein Purification by precipitation - (reply: 2)
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refolding proteins using dialysis - (reply: 1)
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protein refolding - (reply: 1)
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concentrating protein by method other than tff - (reply: 1)
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Protein TAG for affinity purification - (reply: 1)
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BCA Assay and SDS-PAGE Gels Do Not Agree - (reply: 2)
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Quantitating IP'd proteins - (reply: 1)
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help with protein localization study using GFP - (reply: 5)
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Protein shows KD but RNA level is higher - (reply: 4)
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No protein spots in 2D gel - Silver staining! - (reply: 17)
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Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
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Wound healing (scratch) assay - extracellular matrix protein substrate (reply: 1)
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protein crystallography services question - (reply: 1)
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c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
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Problem in expression of protein cloned in pcDNA3 - (reply: 1)
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Recombinant protein aggregation after purification - (reply: 2)
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average total protein content per 293t cell - (reply: 2)
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best and simple method to purify protein - (reply: 38)
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Protein A Sandwich ELISA - Protein A orients Capture Ab-Antigen-primary Ab-detector Ab (reply: 1)
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Localization of kanamycin resistance protein - (reply: 1)
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Are proteases a problem in protein quantification assays? - (reply: 3)
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why my protein precipitates? - (reply: 2)
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serum housekeeping proteins for total protein normalization - (reply: 1)
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serum housekeeping proteins for total protein normalization - (reply: 1)
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protein concentration per well - (reply: 6)
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inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
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Detection of phosphorylation site - (reply: 23)
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help my proteins are disappearing from my PVDF membranes - SDS Page help (reply: 1)
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Sites for protein sequence from DNA sequence - (reply: 2)
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will high-speed centrifuge rupture protein G? - (reply: 1)
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His-tag protein expression - (reply: 1)
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Does Change pH could affect protein interaction - GST pul down (reply: 2)
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Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
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protein extraction - ripa buffer vs urea buffer (reply: 1)
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sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
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problem about fused protein - problem about fused protein (reply: 2)
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Diffused protein in IF stainning - (reply: 2)
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IP my protein from culture supernatant - (reply: 2)
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How to concentrate the protein - (reply: 4)
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Western blot: problem with protein migration - trouble shooting......... please help! (reply: 10)
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High pI proteins - High pI proteins (reply: 4)
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protein overexpression - factors affecting overexpression (reply: 3)
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NUCLEAR PROTEIN PREPARATION - (reply: 1)
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High Gene low protein levels - (reply: 1)
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Lost protein from blot? - (reply: 1)
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293 vs 293TN cells - Which would be beter control in testing against expressed proteins (reply: 1)
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Protein extraction buffer compatible with 2D PAGE - (reply: 4)
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Protein Degradation after overexpression - (reply: 1)
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Protein Extraction from Tissue/ Lysate storage - Several questions regarding lysis buffer usage and storage options (reply: 4)
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e coli strain selection for protein expression - (reply: 2)
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phosphorylation of primers using PNK - (reply: 1)
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IgG SDS-PAGE - (reply: 1)
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SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
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wet or semi-dry transfer for low MW proteins - (reply: 4)
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Problems in upscaling protein expression - (reply: 2)
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Where is the future of the proteomics? - (reply: 5)
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problems with His tagged protein western blots - (reply: 3)
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BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
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Protein aggregates SDS-PAGE - (reply: 5)
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check of amount of proteins on filter after rouge ponceau| - (reply: 1)
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dna loading dye for proteins - (reply: 2)
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too many isoforms expressing proteins - (reply: 2)
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protein optimization - (reply: 3)
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Protein stabilization buffer needed - (reply: 1)
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Freezing eluted His tag protein - (reply: 5)
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molecular cloning troubleshooting - bacterial sequences instead of my protein ! (reply: 3)
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Protein Markers - (reply: 1)
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What if my expressed protein is toxic for the cells... - (reply: 1)
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His-tag protein lost it's tag - (reply: 2)
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AnionExchange problem: proteins do not bind - (reply: 12)
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Dialysis into Lipid - (reply: 2)
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How can I isolate ECM protein from a 6 well dish? - (reply: 4)
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How much protein should be loaded to the column during the purificiation? - Is less more? ;) (reply: 1)
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can someone list some proteasome inhibitors? - co-IP for ubiquitiantion protein (reply: 4)
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GST fusion protein expression - Expression problems using pET41a vector (reply: 1)
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Protein standard curves - (reply: 1)
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Primer for Unkown Protein PCR - How to design primer for unknown protein (reply: 2)
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Western Blot for low expressed protein - (reply: 4)
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radiolabeled protein uptake - (reply: 2)
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small proteins have run ahead of tracking dye - (reply: 1)
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Generation of ChIP grade antibody - Purification of recombinant protein (reply: 3)
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Precipitation of protein - (reply: 3)
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in vitro phosphorylation - (reply: 5)
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Meaning of Recombinant Protein - (reply: 3)
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Measuring DNA, RNA, protein and cell wall synthesis - (reply: 1)
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Equal Protein Quantity - Loading onto Well (reply: 6)
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Desperate! Loss of basic proteins on 2D gels - (reply: 5)
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additional proteins in eluted protein-protein purification of GST tag fusion pro - (reply: 1)
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protein absorbance at 230nm??? - (reply: 1)
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How to look at protein stability? - (reply: 3)
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PEG Precipitation of IgM Antibodies - (reply: 2)
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Fermentas prestained protein marker issue! - (reply: 7)
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reprobing a membrane to detect a protein with the same molecular weight - (reply: 4)
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Expression of fusion proteins - (reply: 1)
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Help with protein extraction buffer problem!? - (reply: 1)
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Purifying Protein Samples to Remove or Reduce SDS? - Need to remove or reduce SDS in protein extract so ELISA will work! (reply: 3)
-
Purification of Protein Extract for ELISA - (reply: 1)
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Protein A-agarose or A-sepharose for immunoprecipitation? - (reply: 3)
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High protease activity in protein extraction - A problem in western blot (reply: 2)
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Number of proteins expressed in cell - (reply: 2)
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Deep sequencing to find gene targets of protein? - (reply: 1)
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Protein at low PH - Strange behavior of His-tagged protein at low PH (reply: 1)
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How to store wet dialysis membranes once opened? - (reply: 4)
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bands of protein seems like it did not move - (reply: 2)
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Constitutive association between two proteins - (reply: 2)
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Elute immunoprecipitated complex with protein G? - (reply: 1)
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protein indentification - protein to be identified on sds page based on MW (reply: 2)
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Help...purify and concentrate protein for IEF - (reply: 1)
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Antibody for Fusion Protein - Antibody/ies against fusion protein (reply: 2)
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Could a 30aa peptides(3×flag+6×his） be expressed by BL21 and be successfu - What is the minimum of the length of expressed protein? (reply: 1)
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NP40 or Triton as membrane protein detergent - (reply: 1)
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What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
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Cloning -> expression -> i see multiple bands :( - overexpression of my 3x flag tagged protein shows 4 bands.. (reply: 5)
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Introduction to x-ray crystallography of proteins - (reply: 1)
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Problem in transfer high MW protein - (reply: 5)
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precipitation of secreted protein from growth media - (reply: 1)
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No IgG Signals in immunostaining - urgent (reply: 2)
-
how to design primer for my n-terminal sequence - hot to design primer for my protein (reply: 2)
-
dimerization or aggregation of my protein! - (reply: 3)
-
GST-tagged protein binds to column but is not eluted - (reply: 1)
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IGg bands on SDS PAGE - (reply: 2)
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Analyzing outer mitochondrial membrane proteins via WB - (reply: 6)
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OKADAIC ACID for protein prep - (reply: 1)
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urea-denatured protein precipitates after dialysis - (reply: 1)
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Expressing 4 proteins in an operon - (reply: 6)
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Protein Transfer Problems from SDS-PAGE Gels - Involves Coomassie Staining (reply: 15)
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Protein concentration for crystallization - (reply: 1)
-
How can I decrease IgG background? - My IgG background is too high!!! (reply: 12)
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TCA and protein percipitation? - (reply: 2)
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Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
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Why does the molecular weight of my protein shift *up* after running it over a H - (reply: 4)
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Protein precipitates upon dialysis - (reply: 5)
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protein separation by filtration - (reply: 3)
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What is the epitope of acetylated tubulin antibody? - (reply: 6)
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Amount of chromatin for IgG control - URGENT! (reply: 1)
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membrane protein aggregation - (reply: 1)
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Loading Equal protein concentrations - Does it make a difference in simple overexpression checking? (reply: 4)
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Problem with transferring proteins from nonreducing SDS-PAGE gel - Help please: proteins are not transferred :-( (reply: 7)
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Protein Expression in stationary phase - (reply: 11)
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Protein Smearing - (reply: 2)
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after purification/storage/buffers... - How not to lose protein after purification (reply: 3)
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His Tag Protein Purification - (reply: 6)
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Non-deacetylable Protein? - Is this possible? (reply: 3)
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Trouble about overexpression by lentiviral transduction - Why no protein of interest expressed!? (reply: 1)
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Amylose bound protein elution - (reply: 1)
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Protein elution from amylose resin - (reply: 1)
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Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
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Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
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Overexpressed protein not detected - Oh, the frustration... (reply: 1)
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Cellular proteins in medium - (reply: 1)
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Western Blot Antibodies for inhibiting protein activity?? - (reply: 2)
-
Detecting phosphorylated Erk1/2 - (reply: 6)
-
save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
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yield vs concentration of protein - (reply: 3)
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cys rich protein expression - (reply: 1)
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insect protein expression - (reply: 1)
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Coupling of protein to NHS activated HP column (GE healthcare)? - (reply: 5)
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protein extraction from tissue, help - (reply: 1)
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Protein extraction from tissue - (reply: 1)
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Transport & storage of protein extracts - (reply: 1)
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Protein bands on the bottom of the gel - (reply: 5)
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problem of membrane protein FPLC - (reply: 1)
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my western blots of membrane protein do not work - (reply: 9)
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IgM quantification - Monoclonal Antibodies (reply: 1)
-
Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
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Protein isolation from skin - (reply: 2)
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How to concentrate my protein? - (reply: 1)
-
small protein, transfer pH - some guidelines for conditions would be great! (reply: 3)
-
Organism specific protein?? - data from maldi-ms (reply: 6)
-
buffer for membrane protein - (reply: 1)
-
Protein cloned in pRK-5 Myc and pcDNA3.1 Myc-His Tag not showing up - I need help for a transient expression system (reply: 3)
-
expression of His-tagged protein in insect cells - (reply: 2)
-
Whole cell lysate including nucelar proteins? - (reply: 3)
-
Protogel Quick-Cast - Has anybody used this to make their protein gels? (reply: 1)
-
Protein concentration and - (reply: 3)
-
membrane protein purification - (reply: 6)
-
VEGF-HEK - Protocol for collecting secreted protein (reply: 1)
-
Protein expression failing - (reply: 1)
-
How does high salt extraction of nuclear proteins work - (reply: 9)
-
Nuclear Protein Extraction - Nuclear Protein Extraction (reply: 1)
-
Dimerization of protein - (reply: 1)
-
Phosphorylated primers - (reply: 5)
-
Isolation of protein crosslinked to plasmid - (reply: 3)
-
Big differences between BCA and Bradford? - Two assays give very different results (reply: 8)
-
Contamination in GST fusion protein purification - (reply: 2)
-
Problem with protein samples - No bands detected at all on gel (reply: 2)
-
Protein localization - Protein localization (reply: 1)
-
moles —SS— groups in a protein calculation - (reply: 3)
-
Purification of scretory protein from bacteria - (reply: 2)
-
express protein with many rare codons - (reply: 1)
-
About GFP- tagged proteins in western blot - (reply: 1)
-
GST-tagged proteins - (reply: 1)
-
WESTERN BLOT OF LARGE AND MINORITY PROTEIN - (reply: 1)
-
lentiviral expression - transefection, low titier, protein expression (reply: 1)
-
No protein bands in SDS-PAGE after sample clean-up steps! HELP! - (reply: 8)
-
Protein Sample Preparation - Western Bloting (reply: 1)
-
defatting of soybean powder for protein isolation - defatting of soybean powder for protein isolation (reply: 3)
-
Protein precipitates during BrCN-sepharose coupling - (reply: 5)
-
Dual cross-linking thoughts - Agents to help analyse proteins indirectly bound to DNA (reply: 14)
-
Blasting Primers for RT-PCR - what's hypothetical proteins? My primers always match to these!! (reply: 2)
-
Protein Standard stability (BCA assay) - (reply: 3)
-
IHC staining of secreted proteins - (reply: 3)
-
Dimeric form of a protein? - I want my monomer! (reply: 2)
-
Ni-NTA his tag protein purification - (reply: 1)
-
Protein Soluble Test - Inclusion Bodies + UREA test? (reply: 3)
-
protein concentration factor - (reply: 3)
-
I want to buy p53 protein - which company? - (reply: 1)
-
recombinant protein quantification by flow cytometry - (reply: 1)
-
Protein identification in 2D gel - (reply: 1)
-
Double Labelling of Same Protein - (reply: 4)
-
problem with lentiviral expression of protein - (reply: 2)
-
For those having problems with running & transfer of high MW proteins - (reply: 1)
-
Protein Size - (reply: 3)
-
why the bands migrate lower in the gel EMSA? - as I increase protein concentration (reply: 1)
-
cloning of non-coding region for protein expression - protein expression (reply: 1)
-
Protein Purification from Qiagen RNeasy Kit - Has anybody done this? (reply: 3)
-
Transfer basic protein to PVDF - (reply: 1)
-
Protein expression - (reply: 4)
-
Protein G vs Protein A for Rabbit Antibodies - (reply: 8)
-
single amino acid mutation effects on protein function - (reply: 1)
-
competition of thrombin and my protein on benzamidine beads - (reply: 5)
-
how to extract protein from agarose? - (reply: 1)
-
Protein expression with T7 promoter - (reply: 1)
-
disappearing high molecular protein and marker,thanks - (reply: 1)
-
I can't resolve 2 proteins (LC3-I and II) in Tricine gels - (reply: 5)
-
Is an enzyme protein of 1100 aa too big to be expressed in E coli - (reply: 4)
-
co-IP of chromatin binding protein - (reply: 2)
-
protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
-
Protein cellular location tool - (reply: 1)
-
Western blotting for proteins in vesicles? - (reply: 2)
-
Protein expression using invitrogen pSecTag2 - (reply: 2)
-
Protein drag in IEF-WB - (reply: 3)
-
His protein expression problem - (reply: 1)
-
need help cloning cdna of a known protein - (reply: 1)
-
protein modelling - (reply: 1)
-
Help with Western Blotting of Keratin Proteins - (reply: 2)
-
Modification of Bradford Assay with SDS - THe attempt to increase sensitivity for a protein with SDS (reply: 1)
-
Mock IgG versus No Antibody - Negative Control - (reply: 6)
-
His-tagged protein runs much faster than expected - 6His-Tagged CcpA protein runs around 26 kD whereas I expect to run 37 (reply: 7)
-
protein going to inclusion bodies - protein going to inclusion bodies (reply: 1)
-
removal of SDS from protein - (reply: 4)
-
prepare sample protein for 2d from E.coli - (reply: 2)
-
Protein won't transfer for Western Blot - (reply: 5)
-
How topurify and measure stability of mAb IgM - (reply: 12)
-
PCR a plasmid protein - (reply: 2)
-
To find a protein's function which MW is 14kD. - (reply: 7)
-
Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
-
protein oxidation - (reply: 1)
-
Protein size and gel percentage - (reply: 7)
-
how to explore protein interactions - (reply: 3)
-
Adventitious chemical binding - Unwanted chemicals in protein solution (reply: 2)
-
Protein precipitation by TCA acid + acetone - (reply: 4)
-
Non Reducing SDS protein ladder?? - (reply: 4)
-
biotinylation of proteins - (reply: 1)
-
Protein Free Blocking Buffers - (reply: 1)
-
More than one protein induced - (reply: 4)
-
Protein aggregates - Monomeric fraction varies with wavelength (reply: 4)
-
Protein aggregates - Monomeric fraction varies with wavelength (reply: 1)
-
protein concentration - can be measured at 280nm (reply: 3)
-
could FLAG-tagged protein be used in the Luc assay? - (reply: 1)
-
Exctinction Co-efficient - glycosylated proteins may be tricky (reply: 3)
-
Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
-
Out of expected protein size in SDS-PAGE - (reply: 10)
-
Does biotinylated proteins will migrate diferently in an SDS than the actual pro - (reply: 7)
-
Mock (IgG) Ct Values - (reply: 3)
-
Storage of overexpressed protein - (reply: 4)
-
HELP_Bradford and protein problems - Different protein concentrations (reply: 1)
-
precipitation of proteins from stained cells - Co-IP failure (reply: 6)
-
Protein present in Western, not ELISA - (reply: 10)
-
problem in the GST fusion protein purification - (reply: 4)
-
how to deal with protein precipitates during dialysis - (reply: 1)
-
reconstitution of membrane proteins into liposomes - (reply: 1)
-
Amount of inoculum? - for protein expression in E.coli (reply: 3)
-
ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)
-
the sensitive amount of protein for western blotting - (reply: 3)
-
Concentrating Protein - 8M Urea, Protein solution, concentrating (reply: 8)
-
Mouse Fibroblasts - need to extract nuclar protein (reply: 4)
-
Qubit Protein Quantitation Issues - Qubit protein quantitation (reply: 2)
-
How to determine final amount of 20 ng protein per WB well - (reply: 8)
-
Protein extraction from plant leaves, bioactivity - (reply: 1)
-
High Molecular weight protein separation - (reply: 2)
-
Secreted protein for loading control - (reply: 2)
-
Protein Disappeareance - (reply: 1)
-
Role of imidazole in lysis buffer - for obtaining recombinant his-tag protein (reply: 4)
-
Immunodepletion of high abundance protein - (reply: 1)
-
How to denaturate protein for ELISA - (reply: 1)
-
2D gel - amount of protein (reply: 1)
-
protein size change after western blot - (reply: 2)
-
Coomassie blue before protein transfer - (reply: 9)
-
protein expression (temperature issue) - trying to express soluble protein at lower temperature (reply: 17)
-
epitope tagging of pcr products - (reply: 1)
-
densitometric analysis for silver stained/coomassie gels? - is it done? staining efficiency same for different proteins? (reply: 6)
-
His tag Protein Elution - Elution Buffer (reply: 2)
-
net charge on protein - (reply: 1)
-
Can GST-tag be separated from GST-fusion protein - (reply: 1)
-
Protein analog - (reply: 1)
-
Increase in protein size - Increase in protein size (reply: 5)
-
How to analyze the ingredients of protein bands in native-PAGE gel? - help (reply: 3)
-
Membrane protein isolation - (reply: 1)
-
Expression of human protein in mice in-vivo - (reply: 3)
-
Help with T7RNAP purification - Cell-free protein expression (reply: 3)
-
step-wise dialysis? - (reply: 11)
-
membrane protein extraction buffer - (reply: 1)
-
Large protein transfer problem - (reply: 8)
-
Measuring protein aggregation using Non-denaturing PAGE - (reply: 3)
-
bacteria lysis - E coli lysis for recombinant protein extraction (reply: 1)
-
Could I denature the proteins that were transferred on a membrane? - (reply: 3)
-
Quantiying bands. - Determination of protein contens (reply: 2)
-
Protein Sample Loading Buffer - (reply: 3)
-
Ni-NTA beads and dialysis tubing - Purification using nickel beads (reply: 4)
-
Tips for Western Blot of Low Molecular Weight Protein (HCV core protein) - (reply: 1)
-
shRNA shows knockdown in protein expression but no change in RT-PCR - (reply: 2)
-
fusion protein construction - (reply: 1)
-
gfp-tagged protein not expressing- help! - (reply: 3)
-
pKa values of the protein - (reply: 1)
-
Plasmids for protein expression in mammalian cells - (reply: 2)
-
affinity purification and proteomics - (reply: 1)
-
Native PAGE w. Western protein marker - (reply: 4)
-
Concentrating Protein with centrifuge concentrator but 50% glycerol!!? - (reply: 2)
-
Lyophilised protein - (reply: 3)
-
Usage of EST data for non-model species in proteomics - (reply: 3)
-
protein stuck - (reply: 3)
-
mRNA versus protein expression. Why inverse correlation? - (reply: 3)
-
A databse linking SNPs to functional consequences? - To the protein (reply: 2)
-
protein is degraded after purification - (reply: 2)
-
Protein qauntitation with nanodrop - SDS and urea (reply: 1)
-
Problem in protein expression - (reply: 2)
-
24 well HeLa Cell Total Protein Concentration? - What would it be (reply: 2)
-
small protein blotting - (reply: 8)
-
What proteins stick to ProteinG on preclearing? - (reply: 3)
-
protein expression - Preparation of starter cultures (reply: 15)
-
help~ how concentrate protein solution in large-scale - (reply: 2)
-
Can my sample be going to the buffer instead of the membrane?? - 17kDa protein not showing in WB, transfer is fine though. (reply: 6)
-
Problem with Protein elution using GST sepharose 4B - (reply: 1)
-
Problem transferring HIV-1 tat protein to a PVDF membrane for western blot - (reply: 5)
-
how to isolate insoluble protein in bacterial induction? - (reply: 4)
-
Protein Concentration assay (Bradford assay) - (reply: 2)
-
protein digestion - (reply: 2)
-
Cleaving Fusion Proteins - (reply: 1)
-
In vivo monitoring leukemia growth - with green fluorescent protein or CFSE (reply: 3)
-
Acrylamide/Bis Solution for Small Protein (14 kDa) - Which Acrylamide/Bis Solution do you use for small proteins (reply: 3)
-
Working gene-coding plasmid does not produce transcript or protein - (reply: 2)
-
Re-dissolving of precipitated protein?? - (reply: 1)
-
Protein purification from Insect media - (reply: 1)
-
sticky proteins in co-IP - (reply: 1)
-
Quality of aliquot for SDS-page in protein purification - When is pure pure enough? (reply: 1)
-
protein/DNA Sequence Alignment - (reply: 1)
-
Purifying a strange native protein - (reply: 6)
-
Protein G Sepharose beads preparation - (reply: 2)
-
Ladder but no protein - (reply: 4)
-
Which test for cytokine production? - by PBMCs stimulated with proteins (reply: 6)
-
MIF protein secretion - Need help in knowing about MIF protein secretion (reply: 2)
-
Improving transient CHO protein expression - (reply: 2)
-
Changing dialysis buffer every half hour and then dialysis ON - protein precipitation prevention (reply: 1)
-
Expression of a GFP-tagged protein - (reply: 2)
-
BCA assay for reducing ends - (reply: 2)
-
BCA assay for reducing ends - (reply: 1)
-
FPLC and Protein Chromatography - Resins, FPLC and protein chromatography (reply: 11)
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Checking For Protein Expression - (reply: 3)
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How do I get rid of this contaminant? - His-tagged protein eluted with 300mM imidazole (reply: 13)
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Detecting Patterns of phosphorylated protein - (reply: 1)
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Is it possible? Harsh sonificiation conditions cause degradation of my protein - (reply: 3)
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Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
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insoluble protein fraction - (reply: 1)
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Hyaluronic Acid Binding Protein 1 (HABP1) - I need some insight to prevent binding of this molecule to itself (reply: 2)
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Newbie question: Stability of protein lystates - Freeze/thaw cycles affect phosphate groups? (reply: 2)
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Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
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extracting protein from trizol - (reply: 2)
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Blue Native Gel - denaturing proteins after transfer? - (reply: 3)
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How much sonication power needed? - protein extraction from cells (reply: 2)
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isolation of membrane associated proteins - (reply: 2)
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Newbie question: how to determine protein concentration after IP? - (reply: 8)
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protein extraction - from cell line in T25 flask (reply: 5)
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Protein labeling calculation - (reply: 4)
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Rabbit IgG purification - Rabbit IgG purification (reply: 5)
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protein extraction using trizol - can we replace guanidine cholride with other denaturants? (reply: 1)
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Determining a protein's antigenic property - (reply: 4)
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Oligo annealing - phosphorylation necessary? - (reply: 4)
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how to design a protein interaction experiment - HELP (reply: 4)
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Desalting protein - (reply: 7)
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Secretory protein? - How to determine if my protein is surely secreted? (reply: 2)
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How to check the translation? - His-tagged protein expression (reply: 8)
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protein complex and siRNA - (reply: 2)
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protein precipitation with TCA for dna extraction? - (reply: 1)
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G Protein Coupled Receptors - (reply: 3)
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Protein structure discovery - (reply: 1)
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TCA precipitation_why protein pellet disapears after centrifugation? - (reply: 2)
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Extraction of protein from sample buffer (+DTT) - (reply: 1)
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Protein solubilisation for iTRAQ protocol/general protein solubilisation - (reply: 2)
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protein precipitation without phenol/chloroform for dna extraction? - (reply: 1)
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protein aggregation problem - (reply: 3)
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protein precipitates out during concentrating it - (reply: 2)
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precipitation during dialysis - (reply: 4)
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Protein glycosylation - How to determine? (reply: 2)
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Protein stability in RIPA buffer at room temp - (reply: 3)
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Protein synthesis inhibition - (reply: 4)
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His tag protein purification - (reply: 3)
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How to extract protein from SDS-PAGE gel? - (reply: 7)
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how to put in evidence protein after IP - (reply: 2)
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GST-fusion protein purification - How to purify it? (reply: 9)
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protein extraction from primary cells - (reply: 1)
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Extracting proteins from cells - (reply: 1)
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EDTA as denaturating agent ?? - for proteins.. (reply: 5)
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amount of proteins in urine - (reply: 12)
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Help_Role of BSA for the protein reconstitution - (reply: 5)
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BCA Protein Assay Problems - (reply: 1)
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Protein lysis buffer - (reply: 4)
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IP low protein and MS - (reply: 1)
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protein degardation during gel filteration - (reply: 2)
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Protein solubility improvements? - any methods? (reply: 5)
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antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
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Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
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How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
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Immunolotting of actin protein coming at higher MW - (reply: 1)
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Isolation of secreted proteins from culture media - (reply: 2)
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proteins stuck at interface between stack and separating gel - (reply: 2)
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Protein loading marker for glycoproteins - (reply: 2)
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Trouble with protein expression in E.coli - No expression of ~ 9-11 kDa protein (reply: 2)
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distinguish protein expression from high homology - (reply: 1)
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Visualizing Small Peptides - Using SDS-PAGE to check for expression of small proteins (reply: 16)
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Lost my proteins ! - (reply: 3)
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Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
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Elution problens - My protein is not eluting in urea 8M buffer ... oh shitt!! (reply: 1)
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Alternative methods to detect protein:protein interaction - (reply: 3)
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protein overexpression question. - (reply: 4)
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Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
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Protein targetting - (reply: 1)
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FCS and Protein assay - (reply: 3)
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Simultaneous BLAST for several proteins - (reply: 4)
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Problems in His-tag protein purification with TALON - (reply: 1)
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protein quantification by bradford - (reply: 3)
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protein extraction from tumor samples containing matrigel? - (reply: 2)
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Extracting Proteins From Soil - (reply: 3)
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Endogenous protein purification - (reply: 2)
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strategy to isolate cDNA and express protein - (reply: 1)
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Amount of protein loaded per well? - (reply: 4)
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signal peptide search - How to find subcellular localization signals in a protein sequence (reply: 1)
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Protein in and out of solution - (reply: 1)
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Problems with my western blotting of LC3, autophagy protein... - (reply: 6)
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taking protein out of membrane - (reply: 1)
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His Tag protein - (reply: 4)
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Contaminating proteins for Fluorescence polarization assay - (reply: 1)
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how to dialysis against acetic acid - (reply: 1)
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protein extraction after cell lysing - (reply: 2)
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Protein extraction from rat aorta - (reply: 3)
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Normal IgG background - (reply: 2)
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Making Recombinant Proteins - (reply: 2)
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sensitive protein quantification assay - BCA not helping (reply: 3)
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sensitive protein quantification assay - (reply: 2)
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GST- protein, batch purification - no protein after elution (reply: 3)
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WB does not correspond to protein array - (reply: 1)
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Nickel IMAC - How to avoid unwanted proteins? - (reply: 4)
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Co-IP problem - Weird IgG band (reply: 1)
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No protein expression - (reply: 3)
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Purifying hydrogen bonded peripheral protein - (reply: 3)
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How to elute this protein?! - (reply: 5)
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Recombinant protein or peptide? - (reply: 1)
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GST-tag protein purification - GST-tag protein purification (reply: 4)
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strength of protein interaction for co Ip - (reply: 1)
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Why were proteins favoured over DNA as the genetic material before 1940? - University Genetics Past EXAM Question (reply: 2)
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Stability of Biotin labelled protein?? - (reply: 1)
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Isolation DNA and protein from a single sample - (reply: 5)
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How to purify a transmembrane protein from others proteins? - (reply: 1)
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Protein structure - (reply: 1)
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Dialysis against PBS of IgG/IgA - a bit confusing (reply: 3)
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simultaneous extraction of nucleic acids and proteins - (reply: 3)
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Protein tags - (reply: 2)
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Could denatured proteins be stored at -20°C (or -80°C) until use (for SDS-PAGE)? - (reply: 3)
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truncation of protein expression - (reply: 1)
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SDS Page Help - using purified protein (reply: 3)
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PROTEIN PRECIPITATION SOLUTION - (reply: 1)
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protein precipitation - (reply: 3)
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GST fusion protein purification - (reply: 1)
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Integral protein question - (reply: 2)
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storage of protein samples - (reply: 1)
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unstable protein expression - (reply: 1)
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DNA vs Protein Stains - Are there any that don't stain BOTH? (reply: 1)
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splice variant encoding same protein - (reply: 1)
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B-per GST fusion protein purification kit - (reply: 2)
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Endogenously Biotinylated Proteins - (reply: 3)
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protein binding site prediction - (reply: 2)
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gradient to separate 1kDa proteins - (reply: 3)
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Protein precipitates out so fast - (reply: 5)
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Protein sticks to Ni beads - Protein doesn't elute from the column/batch etc (reply: 2)
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Principles behind DNA RNA protein isolation and purification - (reply: 2)
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Lysis Buffer for Total Protein Assay - (reply: 1)
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Number of genes and number of proteins in humans - (reply: 1)
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automated protein structure search - (reply: 4)
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"Gentle" IgG elution - (reply: 1)
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Insert phosphorylation upon Klenow? - (reply: 5)
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Protein Conc determination - (reply: 2)
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protein stability - (reply: 3)
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pQE vector protein expression - (reply: 3)
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Importance of Reducing Agent - Apparent protein size on gel (reply: 2)
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Reconstitution of protein standard - (reply: 3)
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Purifying protein with Neutravidin binding peptide tag - Protein purification (reply: 6)
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protein size gradient gel - (reply: 10)
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A story of Urea, dialysis and GST affinity purification... hopefully! - (reply: 1)
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lyophilized protein now looks clear - (reply: 3)
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Playing for keeps - Reusing remaining protein sample (reply: 3)
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isoelectric point of protein - (reply: 1)
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GST-Tagged protein - (reply: 4)
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Effect of sonication on membrane anchored proteins - (reply: 1)
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Methods to determine the oligomeric state of a protein - (reply: 1)
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Problem concerning protein quantitation - (reply: 2)
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Protein concentration - (reply: 4)
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Getting rid of background protein binding on GST beads - (reply: 5)
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denaturing proteins in gel - (reply: 1)
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Protein modification search - (reply: 1)
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Protein degradation - (reply: 2)
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problems with detecting protein expression - protein expression in cell (reply: 4)
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yeast two hybrid - are protein interactions species specific? (reply: 1)
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Problem with protein precipitation - (reply: 1)
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Software to create protein maps - (reply: 1)
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Urea and protein storage - (reply: 6)
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Who know anything about dialysis on protein extractions ? - (reply: 7)
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Problem assessing protein concentration - Protein concentration (reply: 4)
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Protein doesn't bind any column except Ni - (reply: 10)
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Phosphoproteins staining - (reply: 2)
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IgG control for immunopercipitation - (reply: 1)
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quantification of Biotinylated protein ?? - (reply: 3)
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Problems with large protein - (reply: 3)
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protein concentration method - want really high yield (reply: 4)
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protein structure prediction - (reply: 1)
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help! Truncated protein cannot expressed in mammalian cells? - (reply: 5)
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stabilization of purified protein solutions - (reply: 2)
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Rcovery of proteins from gels - (reply: 2)
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Protein much larger on Western blot than predicted - (reply: 12)
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Showed a bigger and broad smear of protein!! - (reply: 4)
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Proteins not running till the end of the gel - (reply: 2)
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The maximum resolution obtained by crystallography? - Can we determine the primary structure of a protein by that? (reply: 3)
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How to purify a protein (PI 8.0) using His tag? - (reply: 4)
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mutation in secreted protein - (reply: 5)
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Recombinant Protein migration - Doubts on protein migration (reply: 5)
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How does the protein fixation on SDS gel work? - (reply: 3)
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Why are my E. coli cells not pellet-able? - when expressing a chimeric recombinant protein (reply: 6)
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SWAT for protein expression - problems with Poly Ala/GC Rich (reply: 1)
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ChIP-CHIP IP vs IgG vs Unbound vs Input - (reply: 2)
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Mammalian protein expression in E. coli - (reply: 1)
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Immunoprecipitation - Multiple proteins from single lysate (reply: 2)
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can beta-catenin be used as a house keeping protein? - (reply: 3)
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WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
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Protein extraction from cell culture media - (reply: 9)
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protein precipitation - (reply: 1)
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brown pigment attached to the protein - (reply: 3)
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Protein extraction from mouse organs - (reply: 6)
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no expression of my protein - (reply: 1)
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confirming nucleotide state of isolated G protein - (reply: 1)
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Tagging a protein with a nucelar export signal - (reply: 2)
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part of protein sequence missing - (reply: 4)
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protein disapeared from gel! - (reply: 11)
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Best storage temperature for samples - serum, protein, tissue, DNA, RNA - Archives (reply: 8)
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Phosphoproteins - (reply: 1)
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Mouse Urinary Protein Quantification - BCA possible (reply: 2)
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How to define the domans in protein sequence - (reply: 3)
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protein purification - (reply: 5)
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protein concentration - how to determine the protein concentration very accurately?? (reply: 2)
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IgM hybridoma protocol - Protocol targeted for isolation of IgM secreting hybridomas (reply: 5)
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Slimy protein lysates - (reply: 3)
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protein band size determination - any software available? (reply: 2)