Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection - (Feb/21/2012 )
Does anyone know if there are differences with using a flat-bottomed vs rounded-bottomed 96-well plate when measuring protein content in unknown lysates?
Would there be any accuracy differences between them and would anyone know the mechanism by which light goes through these plates for measurement purposes?
i used flat for Bradford method
Flat bottom is definitely better for absorbance as the round bottom causes scattering of the light, leading to erroneous readings.