Protocol Online logo
Top : New Forum Archives (2009-): : Biochemistry

protein extraction from tissue, help - (Mar/03/2010 )

Hi there,

I need to extract proteins from a tiny muscle fibre. I'm tending to use the T-PER tissue protein extraction reagent. But I don't know how i could perform the homogenization. Obviously, a hand-hold glass homogenizer won't do since my muscle tissue is very small. Any suggestion on this plz? like sonication?

Many thx,
Jo

-Zhe-

in what volume will you be extracting? there are some very small teflon-glass homogenizers.

you could even homogenize in a eppendorf tube.

-mdfenko-