RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (Aug/11/2011 )
Hi there
I am facing problem related to the resolution of RNA protein complex on NU PAGE gradient gel(4-12%).The organic phase ( RNA-protein complex) shows high resolution whereas the aqueous phase(MS2 MBP tagged RNA ) shows poor resolution on the 4-12% NU PAGE gradient gel after staining with commassie blue.Can anybody guide me how can I get equally good results for both phases.
Wild guess - There are two types of buffers with the NuPAGE 4-12 - MES and MOPS buffer. MES gives you better resolution in the lower molecular weight area and MOPS higher.