storage conditions for purified protein - (Oct/19/2015 )

Hi all,

Can anyone enlighten me on the ideal storing temperature for purified protein?

And how long can it last (based on your experience) in said temperature?

ps: my application for the purified protein is to use on western blotting only. 

Thanks.

It depends on the protein and what form it is in. Lyophilized protein can be stored in the fridge for a while, but lasts almost indefinitely in the freezer. Proteins in buffer will last a while in the freezer (dependent on freeze/thaw), and can last forever at -80, but again this is dependent on freeze/thaw cycles.

Purified protein (such as restriction enzymes) are often stored at -20 in 50% glycerol to prevent freezing, which eliminates freeze/thaw issues.