Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE and Western Blotting

Freezing proteins at -80 - (Jul/20/2011 )


I used RIPA lysis buffer with protease inhibitors to collect cell lysates, and then I clarified the lysates to get protein concentrations.

Then I put the proteins into the -80 freezer to store. However, I did not snap freeze in the liquid nitrogen tank. I am just looking for total protein levels in the lysate. Do you think not snap freezing will be a problem??