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How to increase protein expression from in vitro transcribed mRNA - (Jul/10/2014 )

I'm trying to drive as much expression from an IVT transcript as possible.  What are some modifications I can make to the mRNA that may allow this.  I know about WPRE but are there others that could potentially help?

 

Many thanks,

-A

-Ahrenhase-

Codon optimization?

-bob1-