Protocol Online logo
Top : New Forum Archives (2009-): : Protein Expression and Purification

Quantitating IP'd proteins - (Aug/16/2010 )

I need to quantitate the amount of immunoprecipitated protein for an enzyme kinetics assay. What is the best way to do this?

-meticulous me-

We quantitate our proteins with a Pierce / Thermoscientific BCA Assay Kit. It is fast and works quite good.