Model Protein - Need Advice! - (May/03/2011 )

Hi Bio Community,

I am currently at a bit of a loss for ideas here. What I need is a model protein for my experiments.
Here are the criteria that I hope it can/will posses:
- Phosphorescent/Fluorescent/Colored (any of these properties will suffice)
- Loses above said property when denatured (it looses function, it looses color)
- Can be purchased, and preferably in a lyophilized format (I do not have the capability of growing the protein)
- It is not chemically modified
- Easily soluble and rather stable
- Does not have carbohydrates, ect. attached to it. (glycoproteins)
- Does not need an external factor to visualize (for example B-gal and alkaline phosphatase)

I had originally thought that GFP would be a perfect candidate, but I cannot find anywhere to actually purchase the protein (only the plasmid that encodes it).

If ANYONE has any ideas or suggestions, they would of course be greatly appreciated!

you can get recombinant gfp from roche.

Hmmm... I had hoped that it would be more affordable. 50ul at 1mg/ml for ~$150 is not going to be viable for the screening that I would like to do

Does anyone know of a more affordable option that would still fit my requirements?

PS. Thank you mdfenko for finding that for me.

feldman on Tue May 3 20:51:33 2011 said:

Hmmm... I had hoped that it would be more affordable. 50ul at 1mg/ml for ~$150 is not going to be viable for the screening that I would like to do

Does anyone know of a more affordable option that would still fit my requirements?

PS. Thank you mdfenko for finding that for me.